Name:
Phone:
E-mail:
Message


 


SNE18 | Rossmann-fold NAD(P)-binding domain-containing protein

AS12 2118 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

SNE18 | Rossmann-fold NAD(P)-binding domain-containing protein in the group Antibodies for Plant/Algal  / Photosynthesis  / GreenCut at Agrisera AB (Antibodies for research) (AS12 2118)

DATA SHEET IN PDF

Qty: 
286
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

a part of recombinant SNE18 protein sequence , excluding membrane spanning part, UniProt: Q8GYZ0, TAIR: AT4G31530

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

35.23 | 29.17 kDa (without a transit peptide)

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example
western blot using anti-SNE18 antibodies

From 0.1-1.2 µg of chlorophyll from Arabidopsis thaliana total leaf extract, extracted with buffer A (330 mM sorbitol, 25 mM Tricine pH 7.8, 1 mM EDTA, 10 mM KCl, 0,15 % BSA, 4 mM Na ascorbat (L-ascorbic acid), 7 mM L-cysteine) were separated on 15 % SDS-PAGE gel and blotted 1h to PVDF. Blots were blocked with 10% skimmed non fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1 000 over night at 4ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:10 000 in TTBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 1-3 min.

Courtesy of Dr. Rikard Fristedt, UCLA, USA.

 

Additional information

Related products

Background

Background SNE18 belongs to NAD(P)-binding Rossmann-fold superfamily protein and it functions in steroid biosynthetic process. This protein is located in chloplast.

Product citations

Selected references Amstutz et al. (2020). An atypical short-chain dehydrogenaseÃĒ??reductase functions in the relaxation of photoprotective qH in Arabidopsis. Nat. Plants (2020). doi.org/10.1038/s41477-020-0591-9

Related products: SNE18 | Rossmann-fold NAD(P)-binding domain-containing protein

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS12 1853 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
286 €
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
AS13 2641 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
286 €
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €