showForm Anti-Soy protein antibodies

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Soy protein

AS09 569 | Clonality: Polyclonal | Host: Hen | Reactivity: Glycine max

Soy protein in the group Antibodies for Plant/Algal  / Food Proteins/Allergens at Agrisera AB (Antibodies for research) (AS09 569)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

Glycine max flour protein extract

Host Chicken
Clonality Polyclonal
Purity Affinity purified IgY
Format Liquid in PBS pH 7.2 + 0.075 % sodium azide
Quantity 100 µg
Storage Store at -20°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 2- 5 µl/ml (capture ELISA), 0.1-1 µl/ml (WB)

Reactivity

Confirmed reactivity Glycine max proteins
Predicted reactivity Soybean proteins
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot detection using anti-soy protein antibodies  1 µg (2) and 5 µg (3) of soy protein extract were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-soy protein antibody (AS09 569, 1:10 000, 0.5 µl/5ml, 1h) and secondary anti-chicken (1:25 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with TMA-6 (Lumigen) using a Fuji LAS-3000 CCD (300s, standard sensitivity). Exposure time was 2 min. 


Additional information

Additional information

Antibodies were purified on immobilized soybean proteins.

Related products

Background

Background

Soybean (Glycine max) is a plant from legume family with a broad use in food industry.

Product citations

Selected references

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