AS09 569 | Clonality: Polyclonal | Host: Hen | Reactivity: Glycine max
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Glycine max flour protein extract
|1 µg (2) and 5 µg (3) of soy protein extract were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-soy protein antibody (AS09 569, 1:10 000, 0.5 µl/5ml, 1h) and secondary anti-chicken (1:25 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with TMA-6 (Lumigen) using a Fuji LAS-3000 CCD (300s, standard sensitivity). Exposure time was 2 min.|
Antibodies were purified on immobilized soybean proteins.
Soybean (Glycine max) is a plant from legume family with a broad use in food industry.
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