IKP

Soy protein

Product no: AS09 569

AS09 569 | Clonality: Polyclonal | Host: Hen | Reactivity: Glycine max

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  • Product Info
  • Immunogen:

    Glycine max flour protein extract

    Host: Chicken
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgY in PBS pH 7.2. Contains 0.075 % sodium azide.
    Format: Liquid
    Quantity: 100 g
    Storage: Store at -20C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 2- 5 l/ml (capture ELISA), 0,1-1 l/ml (WB)
  • Reactivity
  • Confirmed reactivity: Glycine max proteins
    Predicted reactivity: Soybean proteins
  • Application Examples
  • western blot detection using anti-soy protein antibodies
    Total soy protein 1 µg (lane 2) and 5 µg (lane 3) were loaded on NuPage gel (Invitrogen) and transferred to the PVDF membrane. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-soy protein antibody (AS09 569, 1:10 000, 0.5 µl/5ml, 1h) and secondary anti-chicken (1:25 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent using a Fuji LAS-3000 CCD (300s, standard sensitivity). Exposure time was 2 min.

    Reactivity of this antibody to egg or milk proteins was not analysed. 
  • Additional Information
  • Additional information: Antibodies were purified on immobilized soybean proteins
  • Background
  • Background:

    Soybean (Glycine max) is a plant from the legume family with a broad use in the food industry.

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