Soy protein
AS09 569 | Clonality: Polyclonal | Host: Hen | Reactivity: Glycine max
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Product Information
Immunogen
Glycine max flour protein extract
Host
Chicken
Clonality
Polyclonal
Purity
Immunogen affinity purified IgY in PBS pH 7.2. Contains 0.075 % sodium azide.
Format
Liquid
Quantity
100 ĩg
Storage
Store at -20°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
2- 5 ĩl/ml (capture ELISA), 0,1-1 ĩl/ml (WB)
Reactivity
Confirmed reactivity
Glycine max proteins
Predicted reactivity
Soybean proteins
Application examples
Application examples
Total soy protein 1 µg (lane 2) and 5 µg (lane 3) were loaded on NuPage gel (Invitrogen) and transferred to the PVDF membrane. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-soy protein antibody (AS09 569, 1:10 000, 0.5 µl/5ml, 1h) and secondary anti-chicken (1:25 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent using a Fuji LAS-3000 CCD (300s, standard sensitivity). Exposure time was 2 min.
Reactivity of this antibody to egg or milk proteins was not analysed.
Total soy protein 1 µg (lane 2) and 5 µg (lane 3) were loaded on NuPage gel (Invitrogen) and transferred to the PVDF membrane. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-soy protein antibody (AS09 569, 1:10 000, 0.5 µl/5ml, 1h) and secondary anti-chicken (1:25 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent using a Fuji LAS-3000 CCD (300s, standard sensitivity). Exposure time was 2 min.
Reactivity of this antibody to egg or milk proteins was not analysed.
Additional information
Additional information
Antibodies were purified on immobilized soybean proteins
Background
Background
Soybean (Glycine max) is a plant from the legume family with a broad use in the food industry.
Product citations
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