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Soy protein

AS09 569 | Clonality: Polyclonal | Host: Hen | Reactivity: Glycine max

Soy protein in the group Antibodies for Plant/Algal  / Food Proteins/Allergens at Agrisera AB (Antibodies for research) (AS09 569)

DATA SHEET IN PDF

Qty: 
250
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen

Glycine max flour protein extract

Host Chicken
Clonality Polyclonal
Purity Affinity purified IgY
Format Liquid in PBS pH 7.2 + 0.075 % sodium azide
Quantity 100 µg
Storage Store at -20°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 2- 5 µl/ml (capture ELISA), 0.1-1 µl/ml (WB)

Reactivity

Confirmed reactivity Glycine max proteins
Predicted reactivity Soybean proteins

Application examples

Application examples western blot detection using anti-soy protein antibodies
Total soy protein 1 µg (lane 2) and 5 µg (lane 3) were loaded on NuPage gel (Invitrogen) and transferred to the PVDF membrane. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-soy protein antibody (AS09 569, 1:10 000, 0.5 µl/5ml, 1h) and secondary anti-chicken (1:25 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent using a Fuji LAS-3000 CCD (300s, standard sensitivity). Exposure time was 2 min.

Reactivity of this antibody to egg or milk proteins was not analysed. 

Additional information

Additional information

Antibodies were purified on immobilized soybean proteins.

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Background

Background

Soybean (Glycine max) is a plant from the legume family with a broad use in the food industry.

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