UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker)

AS05 086  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, C. annuum, C. sativus, F. margarita Swingle, F. arundinacea,  H. vulgare, L. esculentum, L. chilense, Malus x domestica Borkh. c.v. Fuji, M. polymorpha, Medicago truncatula, N. tabacum, O. sativa, P. glauca, Populus sp., S. tuberosum, S. sogarandinum, Triticum |  cellular [compartment marker] of cytoplasm

UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker) in the group Antibodies for Plant/Algal  / Carbohydrates at Agrisera AB (Antibodies for research) (AS05 086)


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Product Information


Recombinant UGPase Q43772 overexpressed and purified from E.coli

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 1500 (IL), 1 : 1000-1 : 5000 (WB)
Expected | apparent MW

51.6 kDa


Confirmed reactivity Arabidopsis thaliana, Arabidopsis halleri, Brassica rapa, Capsicum annuum, Fortunella margarita Swingle, Citrus sinensis, Cucumis sativus, Festuca arundinacea, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum chilense, Malus x domestica Borkh. c.v. Fuji,, Marchantia polymorpha, Medicago truncatula, Mesemryanthenum crystallinum, M.vaginalis, Nicotiana benthamiana,  Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Picea glauca, Populus sp., Solanum lycopersicum, Solanum tuberosum, Solanum sogarandinu, Triticum aestivu, Zea mays
Predicted reactivity Amorpha fruticosa, Bambusa oldhamii, Brachypodium distachyon, Brassica pekinensis, Capsella rubella, Cucumis melo, Eucalyptus grandis, Glycine max, Glycine soja, Gossipium hirsutum, Jatropha curcas, Pinus taeda, Populus tremula, Ricinus communis, Saccharum officinarum, Sorghum bicolor, Theobroma cacao, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in

C. merolae, diatoms

Application examples

Application examples Western blot on cytoplasmic and chloroplastic fractions with anti-UGPase antibodies

UGPase was used as a negative control for cytoplasm contamination in chloroplast fraction. 

Total protein (first 2 lanes to the left) or chloroplast protein (2 lanes to the right) of Arabidopsis thaliana were extracted with 100 mM Tris–HCl, pH 8.0, 150 mM NaCl, 1 mM EDTA, 0.5% Triton X-100, 1 mM DTT, 1 mM PMSF, protease inhibitor cocktail, denatured with Laemmli buffer (62.5 mM Tris–HCl pH 6.8, 2% SDS, 10% glycerol, 100 mM DTT, 0.1% β-mercaptoethanol) at 95°C 5 min and separated on 8% SDS-PAGE and blotted 1h to PVDF, using wet transfer. Blot was blocked with 5% milk for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h/RT with agitation in TBS-T. The antibody solution was decanted and the blot was washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in Calbiochem matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in for 1h/RT with agitation. The blot was washed as above and developed for 10 min with Clarity Western ECL Substrate (Biorad) and visualised with chemiluminescence CCD camera Fluorchem SP (Gel Biosciences). Exposure time was 1-5 minutes.

This antibody is required to be used on chloroplast fraction. There is no signal in the total cell extract. 

Courtesty of Dr. Aleksandra Kwaśnik, Warsaw University, Poland


A 1-year-old greehouse grown plant was dissected into different tissues, which were then used for enzyme assays and immunoblot analyses. Equal amounts of total protein (7.5 μg) were loaded on each lane. SDS-PAGE was run on a 7.5% gel. Immunoblot was done using PVDF transfer membrane. Primary antibodies against barley UGPase were used in 1: 1000 dilution. Secondary antibodies (Rabbit IgG, HRP conjugated) were used at 1:10 000.

ff - female flower, mf - male flower, yl - young leaf, ml - mature leaf, sbk - stem bark, sph - stem phloem and cambium, sxy - stem xylem, rxy - root xylem


western blot using plant anti-UGPase antibodies

15 µg of  total soluble protein  extract from leaves and stems of  Solanum tuberosum (1),  Solanum sogarandinum (2),   Lycopersicum esculentum (3)Lycopersicum chilense  (4) , Arabidopsis thaliana (5) , Cucumis sativus (6)Festuca arundinacea  (7) , Nicotiana tabacum (8) and Capsicum annuum (9)  were separated  on 10% SDS-PAGE and blotted onto nitrocellulose .  After blocking with 5% milk in TBST , blots were incubated with the primary antibody at a dilution of 1:1500 in TBST for 1h  at room temperature. Following incubation and wash steps, blots were incubated with secondary Anti-Rabbit IgG , Alkaline Phosphatase Conjugate for 1 hour at a dilution of 1:40000 . Blots were developed with the alkaline phosphatase detection system using NBT/BCIP.

Courtesy of Bartosz Szabala, Institute of Plant Genetics, Polish Academy of Science .

Additional information

Additional information

Cellular [compartment marker] of cytoplasm, UGPse is a cytoplasmic protein Martz et al. (2002)

This product can be sold containing ProClin if requested.

This antibody detectes 1 ng of UGPase in a western blot and reacts with both cytosolic isoforms only which have similar MW of ca. 52 kDa in Arabidopsis thaliana.

Related products

Related products

AS14 2813 | Anti-UDP-glucose pyrophosphorylase (cytoplasm marker) (Hordeum vulgare
For cytoplasmic marker for Chlamydomonas reinhardtii - please check NAB1

Collection of antibodies to carbohydrate metabolism

Plant protein extraction buffer

Secondary antibodies



UDP-glucose pyrophosphorylase (UGPase, UDPGP) E.C=  is a key enzyme of synthesis of sucrose, cellulose and other saccharides. There are two cytoplasmic isoforms of UGPase-A (which share 94 % identity on amino acid level) and one chloroplastic UGPase-B isoform in Arabidopsis thaliana which share ca. 10-11 % of identity (Kleczkowski et al. 2011).

Product citations

Selected references Pan et al. (2021) Post-Golgi Trafficking of Rice Storage Proteins Requires the Small GTPase Rab7 Activation Complex MON1-CCZ1, Plant Physiology, 2021;, kiab175,
Li et al. (2021) Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit. Hortic Res. 2021 Feb 1;8(1):31. doi: 10.1038/s41438-021-00470-w. PMID: 33518707; PMCID: PMC7848011.
Li et al. (2020). N-terminal acetylation stabilizes SIGMA FACTOR BINDING PROTEIN 1 involved in salicylic acid-primed cell death. Plant Physiol. 2020 Mar 5. pii: pp.01417.2019. doi: 10.1104/pp.19.01417.
Ren et al. (2020). GPA5 Encodes a Rab5a Effector Required for Post-Golgi Trafficking of Rice Storage Proteins. Plant Cell. 2020 Jan 16. pii: tpc.00863.2019. doi: 10.1105/tpc.19.00863.
Ge et al. (2019). The NIN-like protein 5 (ZmNLP5) transcription factor is involved in modulating the nitrogen response in maize. Plant J. 2019 Dec 2. doi: 10.1111/tpj.14628.
Kim et al. (2019). Polyamine uptake transporter 2 (put2) and decaying seeds enhance phyA-mediated germination by overcoming PIF1 repression of germination. PLoS Genet. 2019 Jul 24;15(7):e1008292. doi: 10.1371/journal.pgen.1008292.
Dogra et al. (2019). Oxidative post-translational modification of EXECUTER1 is required for singlet oxygen sensing in plastids. Nat Commun. 2019 Jun 27;10(1):2834. doi: 10.1038/s41467-019-10760-6.
Pontier et a. (2019). The m6A pathway protects the transcriptome integrity by restricting RNA chimera formation in plants. Life Sci Alliance. 2019 May 29;2(3). pii: e201900393. doi: 10.26508/lsa.201900393.
Jones et al. (2019). Arabidopsis JMJD5/JMJ30 Acts Independently of LUX ARRHYTHMO Within the Plant Circadian Clock to Enable Temperature Compensation. Front. Plant Sci., 01 February 2019 |
Lai et al. (2018). Salicylic acid-independent role of NPR1 is required for protection from proteotoxic stress in the plant endoplasmic reticulum. Proc Natl Acad Sci U S A. 2018 May 29;115(22):E5203-E5212. doi: 10.1073/pnas.1802254115.
Shanmugabalaji et al. (2018). Chloroplast Biogenesis Controlled by DELLA-TOC159 Interaction in Early Plant Development. Curr Biol. 2018 Aug 20;28(16):2616-2623.e5. doi: 10.1016/j.cub.2018.06.006.
Hartmann et al. (2018). Subcellular Compartmentation of Alternatively Spliced Transcripts Defines SERINE/ARGININE-RICH PROTEIN30 Expression. Plant Physiol. 2018 Apr;176(4):2886-2903. doi: 10.1104/pp.17.01260.
Howden et al. (2017), Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity. New Phytol. 2017 Jul;215(1):309-322. doi: 10.1111/nph.14540.
Vincent et al. (2017). A genome-scale analysis of mRNAs targeting to plant mitochondria: upstream AUGs in 5' untranslated regions reduce mitochondrial association. Plant J. 2017 Dec;92(6):1132-1142. doi: 10.1111/tpj.13749.
Nagel et al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114.
Schalk et al. (2017). Small RNA-mediated repair of UV-induced DNA lesions by the DNA DAMAGE-BINDING PROTEIN 2 and ARGONAUTE 1. Proc Natl Acad Sci U S A. 2017 Mar 21. pii: 201618834. doi: 10.1073/pnas.1618834114.
Castellano et al. (2016). A pathogenic long noncoding RNA redesigns the epigenetic landscape of the infected cells by subverting host Histone Deacetylase 6 activity. New Phytol. 2016 Sep;211(4):1311-22. doi: 10.1111/nph.14001. Epub 2016 May 12.
Hsu et al. (2016). Super-resolution ribosome profiling reveals unannotated translation events in Arabidopsis. Proc Natl Acad Sci U S A. 2016 Oct 21. pii: 201614788.
Liu et al. (2016). iTRAQ-based quantitative proteomic analysis reveals the role of the tonoplast in fruit senescence. J Proteomics. 2016 Sep 2;146:80-9. doi: 10.1016/j.jprot.2016.06.031.

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