VRN2 | Vernalization protein 2

Name: VRN2 | Vernalization protein 2
Brand: IKP
SKU: AS08 341
Price: 349 EUR
Availability: InStock

AS08 341 | Clonality: Monoclonal | Host: Mouse | Reactivity: Triticum aestivum

VRN2 | Vernalization protein 2 in the group Antibodies Plant/Algal / Plant Developmental Biology / Photomorphogenesis at Agrisera AB (Antibodies for research) (AS08 341)

Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence

Host Mouse

Clonality Monoclonal

Purity Total IgG. Protein G purified purified from Cell culture supernatant.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 10 000 (ELISA), 1 : 1000 (WB)

Expected | apparent MW 23,7 kDa

Reactivity

Confirmed reactivity Triticum aestivum

Predicted reactivity

Triticum monococcum

Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody was able to differentiate VRN-2 from the ZCCT2 protein.

There is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T, monococcum

Background

Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.

Product citations

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immunogen:	KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Mouse
Clonality:	Monoclonal
Purity:	Total IgG. Protein G purified purified from Cell culture supernatant.
Format:	Lyophilized
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Western blot (WB)
recommended dilution:	1 : 10 000 (ELISA), 1 : 1000 (WB)
calculated \| apparent molecular mass [kDa]:	23,7 kDa
Confirmed reactivity:	Triticum aestivum
predicted reactivity:	Triticum monococcum Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody was able to differentiate VRN-2 from the ZCCT2 protein.
additional information (application):	There is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T, monococcum
background:	Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.