Name:
Phone:
E-mail:
Address:






VRN2 | Vernalization protein 2

AS08 341  |  Clonality: Monoclonal  |  Host: Mouse  |  Reactivity: Triticum aestivum

VRN2 | Vernalization protein 2 in the group Antibodies Plant/Algal  / Developmental Biology / Photomorphogenesis at Agrisera AB (Antibodies for research) (AS08 341)
VRN2 | Vernalization protein 2



DATA SHEET IN PDF

Qty: 
337
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence

Host Mouse
Clonality Monoclonal
Purity Total IgG. Protein G purified purified from Cell culture supernatant.
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 10 000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW 23,7 kDa

Reactivity

Confirmed reactivity Triticum aestivum
Predicted reactivity

Triticum monococcum


Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information

VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody was able to differentiate VRN-2 from the ZCCT2 protein.

There is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T, monococcum

Related products

Background

Background

Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.

Product citations

Related products: VRN2 | Vernalization protein 2

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS10 1027  | Clonality: Polyclonal  |  Host: Donkey |  Reactivity: Mouse IgG (...
225 €
AS09 627 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Mouse IgG (H&L...
199 €