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AGO set
Tag Antibody Set 

VRN2 | Vernalization protein 2

AS08 341  |  Clonality: Monoclonal  |  Host: Mouse  |  Reactivity: Triticum aestivum

VRN2 | Vernalization protein 2 in the group Antibodies for Plant/Algal  / Developmental Biology / Photomorphogenesis at Agrisera AB (Antibodies for research) (AS08 341)

PRODUCT INFORMATION IN PDF

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366 €

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Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence

Host Mouse
Clonality Monoclonal
Purity Affinity purified
Format Lyophilized
Quantity 100 ĩg (2x50 ĩg)
Reconstitution For reconstitution add 50 ĩl of sterile water to each tube.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 10 000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW

23.7 kDa

Reactivity

Confirmed reactivity Triticum aestivum
Predicted reactivity

Triticum monococcum

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody was able to differentiate VRN-2 from the ZCCT2 protein.

There is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T. monococcum.

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Background

Background

Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.

Product citations

Selected references

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