ZTL (ADO1) | Zeitlupe (Adagio protein 1)

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AS13 2662 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


23 st
Item No:
AS13 2662

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product information

ZTL  Zeitlupe (Adagio Protein 1) is a component of an E3 ubiquitin ligase complex which is playing a central role in blue light-dependent circadian cycles. Alternative names: Adagio protein 1 (ADO1), F-box only protein 2b (FBX2b), Flavin-binding kelch repeat F-box protein 1-like protein 2, FKF1-like protein 2 (FKL2), LOV kelch protein 1 (LKP1), Clock-associated PAS protein ZTL, ZEITLUPE (ZTL).


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ZTL1 protein sequence, UniProt: Q94BT6, TAIR: AT5G57360

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

65.9 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Hordeum vulgare
Not reactive in

Nicotiana tabacum

Additional information
Selected references

to be added when available, antibody released in September 2014.

application example

western blot using anti-ZTL1 antibodies

80 ug of Arabidopsis thaliana total protein were loaded per lane, samples taken at ZT 12 (12 h after lights are on in a 12 h light : 12 h dark growth regime, 22°C constant temperature). Samples were separated on 10 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blot was blocked in for 1 h in 5% non-fat milk at room temperature (RT) with agitation and includated at 1:1000 dilution of a primary antibody over night in PBST-2% BSA, followed by a secondary antibody incubation at 1:5000 dilution for 1 hour (Agrisera, AS09 602). All washes were done in PBST. Reaction was developed with Thermo SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific).

Courtesy of Dr. Dmitri Nusinow, Danforthcenter, USA

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