ZTL (ADO1) | Zeitlupe (Adagio protein 1)

AS13 2662 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ZTL1 protein sequence, UniProt: Q94BT6, TAIR: AT5G57360

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS, pH 7,4

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 65,9 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Hordeum vulgare
Species of your interest not listed? Contact us

Not reactive in

Nicotiana tabacum

Application examples

application example

80 ug of Arabidopsis thaliana total protein were loaded per lane, samples taken at ZT 12 (12 h after lights are on in a 12 h light : 12 h dark growth regime, 22°C constant temperature). Samples were separated on 10 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blot was blocked in for 1 h in 5% non-fat milk at room temperature (RT) with agitation and includated at 1:1000 dilution of a primary antibody over night in PBST-2% BSA, followed by a secondary antibody incubation at 1:5000 dilution for 1 hour (Agrisera, AS09 602). All washes were done in PBST. Reaction was developed with chemiluminescent detection reagent of highest sensitivity.

Courtesy of Dr. Dmitri Nusinow, Danforthcenter, USA

Additional information

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Plant protein extraction buffer

Secondary antibodies

Background

ZTL Zeitlupe (Adagio Protein 1) is a component of an E3 ubiquitin ligase complex which is playing a central role in blue light-dependent circadian cycles.

Alternative names: Adagio protein 1 (ADO1), F-box only protein 2b (FBX2b), Flavin-binding kelch repeat F-box protein 1-like protein 2, FKF1-like protein 2 (FKL2), LOV kelch protein 1 (LKP1), Clock-associated PAS protein ZTL, ZEITLUPE (ZTL).