ZTL (ADO1) | Zeitlupe (Adagio protein 1)

Name: ZTL (ADO1) | Zeitlupe (Adagio protein 1)
SKU: AS13 2662
Price: 302 EUR
Availability: InStock

AS13 2662 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

ZTL (ADO1) | Zeitlupe (Adagio protein 1) in the group Antibodies Plant/Algal / Plant Developmental Biology / Flowering at Agrisera AB (Antibodies for research) (AS13 2662)

ZTL (ADO1) | Zeitlupe (Adagio protein 1)

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ZTL1 protein sequence, UniProt: Q94BT6, TAIR: AT5G57360

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 65,9 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Hordeum vulgare
Species of your interest not listed? Contact us

Not reactive in Glycine max, Nicotiana tabacum

Application examples

application example

80 ug of Arabidopsis thaliana total protein were loaded per lane, samples taken at ZT 12 (12 h after lights are on in a 12 h light : 12 h dark growth regime, 22°C constant temperature). Samples were separated on 10 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blot was blocked in for 1 h in 5% non-fat milk at room temperature (RT) with agitation and includated at 1:1000 dilution of a primary antibody over night in PBST-2% BSA, followed by a secondary antibody incubation at 1:5000 dilution for 1 hour (Agrisera, AS09 602). All washes were done in PBST. Reaction was developed with chemiluminescent detection reagent of highest sensitivity.

Courtesy of Dr. Dmitri Nusinow, Danforthcenter, USA

Additional information

Background

ZTL Zeitlupe (Adagio Protein 1) is a component of an E3 ubiquitin ligase complex which is playing a central role in blue light-dependent circadian cycles.

Alternative names: Adagio protein 1 (ADO1), F-box only protein 2b (FBX2b), Flavin-binding kelch repeat F-box protein 1-like protein 2, FKF1-like protein 2 (FKL2), LOV kelch protein 1 (LKP1), Clock-associated PAS protein ZTL, ZEITLUPE (ZTL).

Product citations

immunogen:	KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ZTL1 protein sequence, UniProt: Q94BT6, TAIR: AT5G57360
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 1000 (WB)
calculated \| apparent molecular mass [kDa]:	65,9 kDa
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Hordeum vulgare Species of your interest not listed? Contact us
not reactive in:	Glycine max, Nicotiana tabacum
Picture (footer):	application example 80 ug of Arabidopsis thaliana total protein were loaded per lane, samples taken at ZT 12 (12 h after lights are on in a 12 h light : 12 h dark growth regime, 22°C constant temperature). Samples were separated on 10 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blot was blocked in for 1 h in 5% non-fat milk at room temperature (RT) with agitation and includated at 1:1000 dilution of a primary antibody over night in PBST-2% BSA, followed by a secondary antibody incubation at 1:5000 dilution for 1 hour (Agrisera, AS09 602). All washes were done in PBST. Reaction was developed with chemiluminescent detection reagent of highest sensitivity. Courtesy of Dr. Dmitri Nusinow, Danforthcenter, USA
background:	ZTL Zeitlupe (Adagio Protein 1) is a component of an E3 ubiquitin ligase complex which is playing a central role in blue light-dependent circadian cycles. Alternative names: Adagio protein 1 (ADO1), F-box only protein 2b (FBX2b), Flavin-binding kelch repeat F-box protein 1-like protein 2, FKF1-like protein 2 (FKL2), LOV kelch protein 1 (LKP1), Clock-associated PAS protein ZTL, ZEITLUPE (ZTL).