Anti-V-ATPase, A | Vacuolar H+-ATPase subunit A
AS22 4814 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit A, O23654, At1g78900 Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 68.8 | 69 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Chlamydomonas reinhardtii, Brassica napus, Cucumis sativus, Gossypium mexicanum, Hordeum vulgare, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Ostreococcus lucimarinus, Phaseolus aureus, Populus balsamifera, Physcomitrium patens, Solanum lycopersicon, Triticum aestivum, Zea mays
Species of your interest not listed? Contact us
Not reactive in: Chlorella sp., Thermotoga neapolitana - Application Examples
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15 μg/well of microsomal membrane pellet extracted freshly from Arabidopsis thaliana Col-0 with homogenization buffer(350 mMsucrose, 70 mMTris-HCl (pH7.5), 10 % (v/v) Glycerol, 3mM Na2EDTA, 1,5 % (w/v) PVP-40, 4 mMDTT, 1x complete protease inhibitor) and denaturated with Laemmli buffer at 70°C for 5 minutes. Samples were separated on 10% SDS-Page and blottet 1h to PVDF (pore size of 0.2 μm), using wet transfer. Blot was incubated in the primary antibody at a dilution of 1:1000 ON/4°C with agitation.The antibody solution was decanted and the blot was washed 3 times for 10 minutes in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti rabbit IgG horseradish peroxidase conjugated, AS09 602) diluted to 1:10 000 in TBS-T for 2h/RT with agitation. The blot was wahsed as above and developed for 2 min with ThermoScientific SuperSignalTM West Pico PLUS Chemiluminescent subtrate. Exposure time was 20 seconds. Courtesy of both Hanna Bindrich and Nadja Wunsch, Universität Heidelberg, Germany Method for microsomal membrane extraction was based on Wang, Y., and Sze, H. (1985). Chemistry, 260, 10434–10443. Wang et al. (1986). Electrogenic H⁺-pumping pyrophosphatase in tonoplast vesicles of oat roots, pp. 497–502.
- Additional Information
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Additional information: Protocol for isolation of plant vacuolar membranes can be found here. - Background
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Background: V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC=3.6.3.14) is involved in acidification process of various compartements of eucaryotic cell. This protein is coded by VHA-A gene. Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPase subunit A, V-ATPase 69 kDa subunit - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS07 213 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Higher plants including A.comosus, A.thaliana, C.sativus, C. australis R.Br, C.reinhardtii, F. margarita Swingle, H.vulgare, L.esculentum, L.longiflorum, Malus x domestica Borkh. c.v. Fuji, M. truncatula, M.crystallinum, N.tabacum, N.caerulescens, O.sativa, P.hybrida cv. Mitchell, Populus sp., P.vittata, Thellungiella sp., T. aestivum, Z.mays, V. vinifera | Cellular [compartment marker] of tonoplast membrane
AS09 577A | Clonality: Polyclonal | Host: Goat | Reactivity: Higher plants including A.thaliana, A.strigosa, M. truncatula, N. tabacum, S. lycopersicum
This set includes three discounted, well published, compartment marker antibodies of your choice. Benefits of using this antibody set
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