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Tag Antibody Set 

PEPCK | PEP carboxykinase

AS07 241  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for plants A. comosus,  M. giganteus, O. sativa, P. virgatum, P. vulgaris, Saccharum spp., S. alterniflora, S. patens, Z. mays

PEPCK | PEP carboxykinase in the group Antibodies for Plant/Algal  / Global Antibodies at Agrisera AB (Antibodies for research) (AS07 241)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide well conserved in both PEPCK1 and 2 sequences from different plant species including Zea mays Q9SLZ0

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

73 | 78 kDa

Reactivity

Confirmed reactivity Ananas comosus, Miscantus giganteus, Mouse, Oryza sativa, Panicum maximum,, Panicum virgatum, Phaseolus vulgaris, Saccharum spp. hybrid clone C91-301, Spartina alterniflora, Spartina patens, Zea mays
Predicted reactivity

Brassica napus, Chromera velia, Cucumis sativus, Flaveria sp., Lycopersicon esculentum, Medicago sativa, Oryza sativa, Urochloa panicoides, Zoysia japonica, Zea mays

Not reactive in

Arabidopsis thaliana

Application examples

Application examples

Application example

western blot detection using anti-PEPCK antibodies

20 µg of total protein from (1) Arabidopsis thaliana total cell extracted with Protein Extration Buffer, PEB (AS08 300), (2) Phaseolus vulgaris total cell, extracted with PEB, (3) Zea mays total cell extracted with PEB, (4) Miscanthus giganteus total cell extracted with PEB, (5) Panicum virgatum total cell extracted with PEB, (6) Spartina alterniflora  total cell extracted with PEB, (7Spartina patens  total cell extracted with PEB, were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with chemiluminescence detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

Additional information

Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time. Higher protein load 10-20 µg is adviced when working with this antibody.

Antibody can be also used following 2D gel electrophoresis.

This product can be sold containing ProClin if requested.

Related products

Related products

AS07 242 | Anti-GOGAT | glutamine oxoglutarate aminotransferase, rabbit antibodies

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background
Phosphoenolpyruvate carboxykinase (PEPCK, PEP carboxykinase) is an enzyme that catalyses the conversion of oxaloacetate and ATP to phosphoenelpyruvate, carbon dioxide and ADP. PEPCK is encoded by two genes in plants: pck1 and pck2.

Product citations

Selected references Shen et al. (2016). The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice. Rice (N Y). 2016 Dec;9(1):20. doi: 10.1186/s12284-016-0094-5. Epub 2016 May 10.
Aragón et al. (2013). The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles. Plant Cell Rep. Aug 20. (Ananas comosus, western blot detection following 2D gel electrophoresis)

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