Name:
Phone:
E-mail:
Message

AGO1 | Argonaute 1 (Chlamydomonas)

AS14 2776 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardii

AGO1 | Argonaute 1 (Chlamydomonas)  in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / microRNA at Agrisera AB (Antibodies for research) (AS14 2776)

DATA SHEET IN PDF

Qty: 
211
How to cite this product:
Product name, number (Agrisera, Sweden)

Datasheet Product citations Add review

Product Information

Immunogen

recombinant AGO1 of Chlamydomonas reinhardtii

Host Rabbit
Clonality Polyclonal
Purity Protein G purified serum
Format Lyophilized in PBS pH 7.4
Quantity 1 mg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

116.4 | 130 kDa

Reactivity

Confirmed reactivity Recombinant AGO1 of Chlamydomonas reinhardtii

Application examples

Application examples Application example

western blot using anti-AGO1 (Chlamydomonas)

Molecular weight markers (1), recombinant AGO1 of Chlamydomonas reinhardtii (2), recombinant AGO1 of Chlamydomonas reinhardtii (1/2 dilution) (3). Recombinant protein was purified using Ni-NTA Agarose (Qiagen), eluted in 50mM NaH2PO4, 500 mM NaCl and 250 mM imidazole (pH 7.5) and diluted in 1xPBS with 2% SDS. Samples were denatured prior to loading with 5 x loading buffer (350 mM Tris-Cl (pH 7.5), 30% glycerol, 10% SDS and 0.6 M DTT) by heating at 95°C for 5 mins. Samples were separated on NuPAGE 7% Tris-Acetate Protein Gel and blotted 1h to PVDF using tank transfer. Blots were blocked with 1xTBS with 0.1% Tween-20 (TBS-T) and 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for 15 hrs at 4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit Ab) diluted to 1:5000 in TBS-T for 1h at RT with agitation. The blot was washed as above and imaged using the Odyssey Infrared Imager and Image Studio v 2.1.

Courtesy of PhD candidate Claire Agius, University of Cambridge, UK

Additional information

Antibody binds to recombinat AGO1 of Chlamydomonas reinhardtii. Endogenous detection remains to be confirmed.

Related products

Related products

AS09 527 | Anti-AGO1 | argonaute 1, rabbit antibody
AS09 527-ALP | Anti-AGO1 | argonaute 1 (40 µg, ALP-conjugated) 
AS09 527B | Anti-AGO1 | argonaute 1 (40 µg, Biotin conjugated)
AS09 527-HRP | Anti-AGO1 | argonaute 1 (40 µg, HRP-conjugated) 
AS09 527P | AGO1 | argonaute 1 | Blocking peptide
AS13 3682 | Anti-AGO2 | argonaute 2, rabbit antibody
AS09 617 | Anti-AGO4 argonaute 4, rabbit antibody
AS10 671 | Anti-AGO5 | argonaute 5, rabbit antibody
AS10 672 | Anti-AGO6 | argonaute 6, rabbit antibody
AS10 673 | Anti-AGO9 | argonaute 9, rabbit antibody
AS15 3071 | Anti-AGO10 | argonaute 10, rabbit antibody

collection of antibodies to micro RNA

Plant protein extraction buffer

Background

Background

AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Product citations

Related products: AGO1 | Argonaute 1 (Chlamydomonas)

AS14 2797 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardii

286 €
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
AS14 2799 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardii
286 €
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €