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Cat2 | Catalase-2

AS21 4531 | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana, Brassica napus, Oryza sativa

Cat2 | Catalase-2 in the group Antibodies Plant/Algal  / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS21 4531)
Cat2 | Catalase-2



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Product Information

Immunogen

Full length, recombinant catalase 2 from Arabidopsis thaliana catalase-2 UniProt: P25819 TAIR; At4g35090

Host

Rabbit

Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl, of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 56,9 | 55 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brassica napus, Oryza sativa
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western Blot using anti-Plant Catalase 2 antibodies

Sample description 1- 15 µg of Arabidopsis thaliana Col-0 extract 2- 15 µg of Arabidopsis thaliana cat2 extract (replicate 1) 3- 15 µg of Arabidopsis thaliana cat2 extract (replicate 2)
MW markers: from Thermo Fisher (Product #26619)

15 µg of total protein from 4-weeks-old Arabidopsis thaliana plants extracted with Tris-HCl 50 mM pH 7.8, 0.1 mM EDTA, 0.2 % (V/V) Triton X-100; prepared in 0.063 M Tris-HCl buffer, pH 6.8, containing 2 % sodium dodecyl sulfate (SDS; w/v), 10 % glycerol (v/v), 0.006 % bromophenol blue (w/v) and 10 mM DTT; and denatured at 95 °C for 5 min. Proteins were separated on 12 % SDS-PAGE and blotted 1 h to PVDF using semi-dry transfer. Blots were blocked with 3 % (w/v) skimmed milk powder diluted in TBS-T O/N at 4 °C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2.000 for 1 h at RT with agitation in TBS-T + 3 % (w/v) of skimmed milk powder. The antibody solution was decanted and the blot was washed 3 times for 10 min in TBS-T + 3 % (w/v) of skimmed milk powder at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, Product No: AS09 602 lot 2012) diluted to 1:10.000 in TBS-T + 3 % (w/v) of skimmed milk powder for 1 h at RT with agitation. The blot was washed as above but with TBS and developed for 5 min with chemiluminescent detection reagent. Exposure time was 20 seconds.

Courtesy of Dr. Luisa M. Sandalio, CISIC, Spain

Additional information

Related products

Background

Background Cat2 (Catalase 2)  is an enzyme which occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.

Product citations

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