IKP

ABA | Abscisic acid (C1) (1 mg)

Product no: AS09 422

AS09 422 | Clonality: Polyclonal | Host: Rabbit

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  • Product Info
  • Immunogen:

    BSA-conjugated abscisic acid (C1) via C1 carboxyl group

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Total IgG. Protein G purified in PBS.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 70 µl of dest, water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Radioimmunoassay (RIA)
    Recommended dilution: 2-10 µl/15 ml; Specific information about dilution is going to be included on the vial
  • Reactivity
  • Confirmed reactivity: Abscisic acid (C1)
    Predicted reactivity: Abscisic acid (C1)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: ABA | Abscisic acid (C1) (1 mg)
    Additional information (application): For ELISA kit - please inquire
  • Background
  • Background:

    Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Assay parameters of anti-abscisic acid (C1) antibodies in ELISA 

    Titre 1: 5300
    Unspecific binding 2.8 %
    Midrange (B/Bo=50%) 275 pmol
    Detection limit 7.8 fmol, 2pg
    Linear range of measurment    15-5000 fmol       
    Intraassay variability 4.3 %
    Intraassay variability 5.2 %
    Amount of tracer per assay    12.4 ng

     

     

     

     

     

     

     

     

    ELISA graph for anti-abscisic acid (C1) antibodies

    Molar cross-reactivities of different abscisic acids and related compunds with anti-abscisic acid (C1) antibodies

    Data presented are expressed as percentage ratio of molar concentrations of appropriate ABA and competitor producing 50% inhibition in ELISA.

    Compund Cross-reactivity (%)
    (±)-cis,trans-abscisic acid 100
    (-)-cis,trans-abscisic acid  47
    (+)-cis,trans-abscisic acid 52
    (±)-trans,trans-abscisic acid 3.27
    (+)-trans,trans-abscisic acid 1.06
    (±)-cis,trans-abscisic acid methyl ester 217
    (±)-cis,trans-abscisic acid glucosyl ester 115
    phaseic acid 0
    diphydrophaseic acid 0.09
    xanthoxin 0.05
    lunularic acid 0
    violaxanthin- 0
    farnesol 0
    vomifoliol 0

     

    Sample preparation

    Processing of plant extracts required prior ELISA analysis may vary from plant to plant and will depend on the actual research objective. In most cases, the removal of pigemtns and lypophilic material by C18 reversed phase chromatography and subsequently by combined DEAE cellulose-reversed phase C18 is all that required. the ABA samples have to be methylated by diazomethane before ELISA analysis.

     

     

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