Name:
Phone:
E-mail:
Message


 


ADH | Alcohol dehydrogenase (hypoxia marker)

AS10 685 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana, Oryza sativa, Phaseolus vulgaris

ADH | Alcohol dehydrogenase (hypoxia marker) in the group Antibodies for Plant/Algal  / Environmental Stress / Hypoxia at Agrisera AB (Antibodies for research) (AS10 685)

DATA SHEET IN PDF

Qty: 
373
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Customer reviews

Product Information

Immunogen

KLH-conjugated peptide derived from available ADH sequences including Arabidopsis thaliana P06525, At1g77120

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW

42 | 42 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Fragaria vesca, Oryza sativa, Phaseolus vulgaris
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in Allyl alcohol dehydrogenase of Nicotiana tabacum, accession 75206691 and in Chlamydomonas reinhardtii.

Application examples

Application examples

Application example

western blot using anti-plant ADH antibodies

 

20 µg of total protein from Arabidopsis thaliana seedlings (0-4-8 hours of anoxic treatment with aerobic control) of WT Col-0 and adh mutant extracted with an SDS Extraction Buffer (60mM Tris-HCl pH 8.0, 2% SDS, 1,5% Sucrose) were separated on XT CRITERION 10%Bis-Tris (BioRad) SDS-PAGE and blotted 1h to PVDF. Blot was blocked immediately in milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the anti-ADH antibodies at a diluition of 1: 3000 in milk in TBS-T for 3h at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG HRP conjugated from Agrisera, AS09 602) diluited 1:20 000 in milk in TBS-T for 50 min at RT and then washed as above and developed for 2 min with chemiluminescent detection reagent. Images of the blot were obtained using BioSpectrum AC Imaging System (UVP). Exposure time was 10 min The arrow indicates ADH (42kDa, as expected) . There is a cross reacting band in Arabidopsis thaliana between 50-70 kDa. The large band in the right corner of the membrane is likely a staining artefact.

western blot detection of alcohol dehydrogenase in rice

 

20 µg of total protein from Orysa sativa coleoptiles (3-4-5-6 days of germination under aerobic and anoxic conditions) extracted with an SDS Extraction Buffer (60mM Tris-HCl pH 8.0, 2% SDS, 1,5% Sucrose) were separated on XT CRITERION 10% Bis-Tris (BioRad) SDS-PAGE and blotted 1h to PVDF. The blot was blocked immediately in milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the anti-ADH antibodies at a diluition of 1: 3000 in milk in TBS-T for over night with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG HRP conjugated from Agrisera, AS09 602) diluted 1:20 000 in milk in TBS-T for 50 min at RT and then washed as above and developed for 2 min with chemiluminescent detection reagent. Images of the blot were obtained using BioSpectrum AC Imaging System (UVP). Exposure time was 10 min. The band corresponds to ADH (41 kDa).

Courtesy Dr. Eleonora Paparelli,Scuola Superiore Sant'Anna, Italy

Additional information

Additional information This product can be sold containing ProClin if requested.

Related products

Related products

AS10 691 | Anti-PDC | pyruvate decarboxylase, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

Alcohol dehydrogenase (ADH) is an enzyme playing a crucial role in the fermentative metabolism in plants subjected to low oxygen stress. It is known to be synthesized preferentially under low oxygen conditions.

Product citations

Selected references Gil-Monreal et al. (2019). ERF-VII transcription factors induce ethanol fermentation in response to amino acid biosynthesis-inhibiting herbicides. J Exp Bot. 2019 Aug 6. pii: erz355. doi: 10.1093/jxb/erz355.
Bui et al. (2019). Conservation of ethanol fermentation and its regulation in land plants. J Exp Bot. 2019 Feb 28. pii: erz052. doi: 10.1093/jxb/erz052.
De la Rosa et al. (2019), A dicistronic precursor encoding miR398 and the legume-specific miR2119 coregulates CSD1 and ADH1 mRNAs in response to water deficit. Plant Cell Environ. 2019 Jan;42(1):133-144. doi: 10.1111/pce.13209.
Giuntoli et al. (2014). A trihelix DNA binding protein counterbalances hypoxia-responsive transcriptional activation in Arabidopsis. PLoS Biol. 2014 Sep 16;12(9):e1001950. doi: 10.1371/journal.pbio.1001950. eCollection 2014.

Related products: ADH | Alcohol dehydrogenase (hypoxia marker)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
195 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €
AS10 691  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:&...
373 €