Agrisera Super Deal


Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)

- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

HSP70 | Salmonid heat shock protein 70, Affinity purified

AS05 061A  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Salmon

HSP70 | Salmonid heat shock protein 70, Affinity purified in the group Human/Animal Antibodies / Fish Proteins at Agrisera AB (Antibodies for research) (AS05 061A)


381 €

Info: Add review
product information

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.


KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 B5X4Z3.

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 200 µg
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunohistochemistry (IHC), Immunoprecipiation (IP), Western blot (WB)
Related products

Collection of antibodies to fish related targets

Secondary antibodies

Additional information

Immunohistochemistry experiments have been done on salmon tissue treated with hydrated autoclaving of formalin fixed sections (unpublished results)

application information
Recommended dilution 5 ĩg of antibodies in reaction mixture (IP), 1 : 100 (IHC), 1 : 15 000 (WB)
Expected | apparent MW

70 kDa

Confirmed reactivity Rainbow trout (Oncorhynchus mykiss)
Predicted reactivity

Salmo salar (Atlantic salmon), Salvelinus fontinalis (Brook trout)

Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

This antibody is recognizing the inducible Hsp70 in salmon but not the constitutive.

Selected references Gallant el al. (2017). Physiological responses to a short-term, environmentally realistic, acute heat stress in Atlantic salmon, Salmo salar. FACETS.
Lewis et al. (2016). Different Relationship between hsp70 mRNA and hsp70 Levels in the Heat Shock Response of Two Salmonids with Dissimilar Temperature Preference. Front Physiol. 2016 Nov 7;7:511. eCollection 2016.
Curie et al. (2008). β‐Adrenergic Stimulation Enhances the Heat‐Shock Response in Fish. Physiol & Bioch. Zoology 4:414-425.

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