HSP70 | Salmonid heat shock protein 70, Affinity purified

AS05 061A | Clonality: Polyclonal | Host: Rabbit | Reactivity: Salmon

Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 UniProt: B5X4Z3.

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 5 µg of antibodies in reaction mixture (IP), 1 : 100 (IHC), 1 : 15 000 (WB)

Expected | apparent MW

70 kDa

Reactivity

Confirmed reactivity Oncorhynchus mykiss (Rainbow trout), Danio rerio (Zebrafish)

Predicted reactivity

Coregonus clupeaformis, Oncorhynchus mykiss, Salmo salar (Atlantic salmon), Salvelinus fontinalis (Brook trout)

Not reactive in Gasterosteus aculeatus

Application examples

Reactant: Salmonid fish

Application: Western Blotting

Pudmed ID: 27872596

Journal: Front Physiol

Figure Number: 5C,D

Published Date: 2016-11-23

First Author: Lewis, M., Götting, M., et al.

Impact Factor: 4.141

Open Publication

Time course of heat shock induced hsp70 mRNA and protein synthesis in Arctic charr. Relative quantities (mean ± SEM; n = 7) in (A) gills and (B) liver. Relative quantities of hsp70 are based on hsp/?-actin ratios in the western blots. Representative examples of western blots for gills are given in (C) and liver in (D). Whenever ANOVA or Kruskal-Wallis on ranks [parametric (p) or non-parametric (n-p), respectively] indicated statistically significant differences post-hoc testing (Dunnett's method) was conducted. A letter above a symbol indicates that the levels at that time point are significantly different from levels before the heat shock (p < 0.05).

Additional information

Immunohistochemistry experiments have been done on salmon tissue treated with hydrated autoclaving of formalin fixed sections (unpublished results)

This antibody is recognizing the inducible Hsp70 in salmon but not the constitutive

Background

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.

Product citations

Selected references Mottola et al. (2020). Comp Biochem Physiol A Mol Integr Physiol. 2020 Feb;240:110629. doi: 10.1016/j.cbpa.2019.110629.
Gallant el al. (2017). Physiological responses to a short-term, environmentally realistic, acute heat stress in Atlantic salmon, Salmo salar. FACETS.
Lewis et al. (2016). Different Relationship between hsp70 mRNA and hsp70 Levels in the Heat Shock Response of Two Salmonids with Dissimilar Temperature Preference. Front Physiol. 2016 Nov 7;7:511. eCollection 2016.
Curie et al. (2008). β‐Adrenergic Stimulation Enhances the Heat‐Shock Response in Fish. Physiol & Bioch. Zoology 4:414-425.

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