HSP70 | Salmonid heat shock protein 70, Affinity purified

AS05 061A | Clonality: Polyclonal | Host: Rabbit | Reactivity: salmon


product information
Background		Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.
Immunogen		KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 B5X4Z3.
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Affinity purified serum in PBS, pH 7.4
Format		Lyophilized in PBS pH 7.4
Quantity		200 µg
Reconstitution		For reconstitution add 200 µl of sterile water
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Immunohistochemistry (IHC), Immunoprecipiation (IP), Western blot (WB)
Related products		Collection of antibodies to fish related targets Secondary antibodies
Additional information		Immunohistochemistry experiments have been done on salmon tissue treated with hydrated autoclaving of formalin fixed sections (unpublished results)

application information
Recommended dilution		5 µg of antibodies in reaction mixture (IP), 1 : 100 (IHC), 1 : 15 000 (WB)
Expected \| apparent MW		70 kDa
Confirmed reactivity		Rainbow trout (Oncorhynchus mykiss)
Predicted reactivity		Salmo salar (Atlantic salmon), Salvelinus fontinalis (Brook trout)
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		This antibody is recognizing the inducible Hsp70 in salmon but not the constitutive.
Selected references		Gallant el al. (2017). Physiological responses to a short-term, environmentally realistic, acute heat stress in Atlantic salmon, Salmo salar. FACETS. Lewis et al. (2016). Different Relationship between hsp70 mRNA and hsp70 Levels in the Heat Shock Response of Two Salmonids with Dissimilar Temperature Preference. Front Physiol. 2016 Nov 7;7:511. eCollection 2016. Curie et al. (2008). β‐Adrenergic Stimulation Enhances the Heat‐Shock Response in Fish. Physiol & Bioch. Zoology 4:414-425.

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