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Tag Antibody Set 

HSP70 | Salmonid heat shock protein 70, Affinity purified

AS05 061A  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Salmon

HSP70 | Salmonid heat shock protein 70, Affinity purified in the group Antibodies for Human/Animal  / Fish Proteins at Agrisera AB (Antibodies for research) (AS05 061A)

PRODUCT INFORMATION IN PDF

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381 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 UniProt: B5X4Z3.

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 200 µg
Reconstitution For reconstitution add 200 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunohistochemistry (IHC), Immunoprecipiation (IP), Western blot (WB)
Recommended dilution 5 µg of antibodies in reaction mixture (IP), 1 : 100 (IHC), 1 : 15 000 (WB)
Expected | apparent MW

70 kDa

Reactivity

Confirmed reactivity Rainbow trout (Oncorhynchus mykiss)
Predicted reactivity

Salmo salar (Atlantic salmon), Salvelinus fontinalis (Brook trout)

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

Immunohistochemistry experiments have been done on salmon tissue treated with hydrated autoclaving of formalin fixed sections (unpublished results)

This antibody is recognizing the inducible Hsp70 in salmon but not the constitutive.

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Background

Background

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.

Product citations

Selected references Gallant el al. (2017). Physiological responses to a short-term, environmentally realistic, acute heat stress in Atlantic salmon, Salmo salar. FACETS.
Lewis et al. (2016). Different Relationship between hsp70 mRNA and hsp70 Levels in the Heat Shock Response of Two Salmonids with Dissimilar Temperature Preference. Front Physiol. 2016 Nov 7;7:511. eCollection 2016.
Curie et al. (2008). β‐Adrenergic Stimulation Enhances the Heat‐Shock Response in Fish. Physiol & Bioch. Zoology 4:414-425.

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