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Anti-GLU1 | Ferredoxin-dependent glutamate synthase 1, chloroplastic/mitochondrial
AS22 4708 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana GLU1 protein sequence, UniProt: Q9ZNZ7, TAIR: At5g04140 Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 176.75 | 130 kDa (due to N-terminal or C-terminal processing) - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Physcomitrium patens, Synechocystis sp.
Species of your interest not listed? Contact usNot reactive in: Euglena gracilis, Lemna minor - Application Examples
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Samples:
1- 20 µg of Arabidopsis thaliana whole leaf extract; col-0 (wildtype)
2- 20 µg of Arabidopsis thaliana whole leaf extract; col-0 (wildtype)
3- 20 µg of Arabidopsis thaliana whole leaf extract; fd-gogat1/glu1/orb1: SALK_011035C
4- 20 µg of Arabidopsis thaliana whole leaf extract; fd-gogat1/glu1/orb1: SALK_011035C
Mark: MW markers; PageRuler Prestained Protein Ladder, Thermo Fisher Cat. #26616
20 ug/well of total protein extracted freshly from Arabidopsis thaliana leaf tissue. Extract buffer components were: 100mM MOPS buffer pH 7.6, 100 mM NaCl, 5% SDS, 0.5% Beta-ME, 10% Glycerin and denatured with exact buffer components at 65℃ for 3 minutes. Samples were separated on 10% SDS-PAGE and blotted for 30 minutes to PVDF (pore size of 0.45 µm), using semi-dry transfer at room temperature (Bio-Rad Trans-Blot Turbo Transfer system). Blot was blocked with 6% BSA for 2 h/RT with agitation (25 rpm). Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight at 4 ℃ with agitation (25 rpm). The antibody solution was decanted, and the blot was rinsed briefly, then washed once for 15 min and 2 times for 5 min in TBST at room temperature with agitation ON (25 rpm). Blot was incubated in matching secondary antibody (HRP Goat Anti-Rabbit IgG) diluted to 1: 3000 for 1 h/RT with agitation (25 rpm). The blot was washed as above and developed with a following chemiluminescent detection reagent: SuperSignal West Pico PLUS Chemiluminescent Substrate, Thermo Scientific. Exposure time was 30 seconds.
Note: Blocking with 5 % 1h/RT nonfat milk may decrease background signal.
Courtesy of Dr. Jake Brunkard, University of Wisconsin-Madison, USA
- Background
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Background: GLU1 (Ferredoxin-dependent glutamate synthase 1) is a chloroplastic enzyme (EC:1.4.7.1 ) essential for plant growth, acting as the primary isoform for reassimilating ammonia released during photorespiration in leaves. It catalyzes the conversion of glutamine and 2-oxoglutarate into two glutamate molecules using reduced ferredoxin, also playing key roles in primary nitrogen assimilation, chlorophyll biosynthesis, and iron homeostasis. Alternative name: Fd-GOGAT 1
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS08 295 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] | Arabidopsis thaliana, Eragrostis tef, Gracilaria gracilis (red algae), Gracilaria lemaneiformis, Leptodictyum riparium (Hedw.) Warnst (moss), Medicago truncatula, Physcomitrella patens, Pinus strobus, Spinacia oleracea, Solanum lycopersicum, Triticum aestivum, Zea mays
Benefits of using this antibody
AS08 310 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, C. reinhardtii, red alga Gracilaria gracilis, Medicago sativa, diatom Thalassiosira sp., P. tricornutum Bohlin, P. yunnanensis Dode, P. notoginseng, S. lycopersicum, S. tuberosum
