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Tag Antibody Set 

GLN1 GLN2 | GS1 GS2 glutamine synthetase global antibody

AS08 295 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] | A.thaliana, E.tef, G.gracilis (red algae), G. lemaneiformis, M.truncatula, P.patens, P. strobus, S.oleracea, S.lycopersicum, Z.mays

GLN1 GLN2 | GS1 GS2 glutamine synthetase global antibody in the group Antibodies for Plant/Algal  / Global Antibodies at Agrisera AB (Antibodies for research) (AS08 295)

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Datasheet Product citations Customer reviews

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from a wide range of available sequences including all isoforms of Arabidopsis thaliana GLN1-1,1-2,1-3 and 1-4, (At5g37600, At1g66200, At3g17820, At5g16570)

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

39-40 kDa (GLN1,cytoplasmic form) , 44-45 kDa (GLN2, chloroplastic form)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Eragrostis tef, Gracilaria gracilis (red algae), Gracilaria lemaneiformis, Medicago truncatula, Physcomitrella patens, Pinus strobus, Spinacia oleracea, Solanum lycopersicum, Zea mays
Predicted reactivity

Brachypodium distachyon, Brassica napus, Camellia sinensis, Citrus clementina, Cucumis melo, Daphnia magna, Datisca glomerata, Emiliania huxleyi, Eucalyptus grandis, Gazania splendens, Genlisea aurea, Glycine max, Helianthus annuus, Hordeum vulgare, Oryza sativa, Panax quinquefolius, Phaseolus angularis, Phytophthora cinnamomi, Populus trichocarpa, Saccharum officinarum, Securigera parviflora, Solanum lycopersicum, Solanum tuberosum, Stevia rebaudiana, Theobroma cacao, Triticum aestivum, Zea mays, Vitis labrusca

GLN1 dicots including: Brassica napus, Phaseolus vulgaris, monocots including: Hordeum vulgare, Oryza sativa, trees: Pinus sylvestris, Populus sp., Zosteria marina

GLN2 dicots including: Brassica napus, Glycine max, Phaseolus vulgaris, monocots including: Triticum aestivum, Oryza sativa

GLN3: Zea mays,

GLN1 in algae: Chlamydomonas reinhardii

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

Western blot using anti-GS (global Gln1/Gln2) antibodies


10 µg of total protein extracted freshly from Arabidopsis thaliana wt leaf tissue (Atn non-senescent leaves), Arabidopsis thaliana wt leaf tissue (Ats senescent leaves), Pinus strobus needle tissue (PSA-J)  with 1 M Tris-HCl, pH 6.8, 10 % SDS, 15 % sucrose, 0.5 DTT and denatured at 75°C for 5 min. were separated on 10 % Bis-Tris Nupage Novex gel (120 V/45 min. using MES buffer system) and blotted 30 min. to PVDF. Blot was blocked with 5 % non-fat milk 45 min./RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h/RT with agitation in TBS with 2 % non-fat milk or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice for 10 min. in TBS at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:75 000 in for 1h/RT with agitation. The blot was washed as above and developed using chemiluminescent detection. Exposure time was 26.5 seconds.

Courtesy of Dr. Christine Yao-Yun Chang and the Ensminger lab, University of Toronto, Canada

Additional information

Additional information

The antibody will recognize both, cytoplasmic and chloroplastic forms of the GS enzyme.

Related products

Related products

AS08 295PRE | GLN1 GLN2 | GS1 glutamine synthetase, pre-immune serum

AS08 296 GLN | GS2, chloroplastic form of glutamine synthetase

collection of antibodies to proteins involved in nitrogen metabolism

plant and algal protein extraction buffer

secondary antibodies

Background

Background

Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN1 is highly abundant in the vascular elements of roots nodules, flowers and fruits, functioning in the assimilation of ammonium and the biosynthesis of glutamine for nitrogen transport. GLN2 is encoded by a single gene and is highly abundant in leaf mesophyll chloroplasts. Here GLN functions in the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts

Product citations

Selected references Wang et al. (2018). Response of Gracilaria lemaneiformis to nitrogen deprivation. Algal Research Volume 34, September 2018, Pages 82-96.
Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.
Silva et al. (2015). Possible role of glutamine synthetase of the prokaryotic type (GSI-like) in nitrogen signaling in Medicago truncatula. Volume 240, November 2015, Pages 98–108.
Podgórska et al. (2013). Long-term ammonium nutrition of Arabidopsis increases the extrachloroplastic NAD(P)H/NAD(P)+ ratio and mitochondrial reactive oxygen species level in leaves but does not impair photosynthetic capacity. Plant Cell Environ. April 10.
Brouwer et al. (2011) TheImpact ofLightIntensity onShade-InducedLeaf Senescence. Plant Cell Environ. Dec. 15 (ahead of print).
Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).

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