AP2 | Floral homeotic protein APETALA 2

Product no: AS12 2609

AS12 2609 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Oryza sativa

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  • Data sheet
  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from AP2 sequence of Arabidopsis thaliana, UniProt: P47927, TAIR: At4g36920

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000-1 : 6000 (WB)
    Expected | apparent MW: 47,8 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Brassica napus, Oryza sativa
    Predicted reactivity: Arabis alpina, Lepidium appelianum
    Species of your interest not listed? Contact us
    Not reactive in: Nicotiana tabacum, Vitis vinifera
  • Application Examples
  • Application example

    western blot using anti-AP2 antibodies
    1 µg of total protein from Oryza sativa lines grown under drought stress conditions and over expressing protein DREB (AP2 domain, 285 aa, 31.35 kDa) (lines 1-5) and purified 100 ng of DREB (line 6), were separated on 12.5 % SDS-PAGE and blotted 4h to Nitrocellulose membrane. Blots were blocked with blocking agent 5% BSA or 1X TBS, 0.1% Tween-20 with 5% w/v non-fat dry milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 6 000 for 1h at RT or overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 x 5 min in 1XTBST at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:8000 in for 1h at RT or max. 2h with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was preferred between 20 to 30 seconds or max. 1 min.

    Courtesy of Dr. Sunita Yadav, Indian Institute of Technology Kharagpur, India
  • Additional Information
  • Additional information (application):
    This antibody was used in western blots on Lepidium appelianum and Lepidium campestre AP2 Protein expressed in E. coli cells. Applied dilution was 1: 6000.

    Contains 0.02 % sodium azide.
  • Background
  • Background:

    AP2 (floral homeotic protein APETALA 2) is a transcription factor involved in the specification of floral organ identity, establishment of floral meristem identity and controlling seed mass. 

  • Product Citations
  • Selected references: Wang et al. (2021). Brassinosteroids inhibit miRNA-mediated translational repression by decreasing AGO1 on the endoplasmic reticulum. J Integr Plant Biol. 2021 May 21. doi: 10.1111/jipb.13139. Epub ahead of print. PMID: 34020507.
  • Protocols

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