WUS | WUSCHEL protein

Product no: AS11 1759

AS11 1759 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    KLH-conjugated peptide derived from Arabidopsis thaliana WUSCHEL protein sequence, UniProt: Q9SB92, TAIR:At2g17950

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Liquid
    Quantity: 50 g
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 33,1 | kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Arabidopsis thaliana
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application information

    western blot using anti-WUS antibodies
    Plant material: Col-0 wild type (1), pWUS::WUS-GFP (2), expression level comparable to wild type pUbiquitin::mCherry-GR-WUS (3), over-expresses the wuschel protein and also has ectopic expression. Used as a positive control for the westerns. 200mg of 5-7 day old seedling were extracted with 600 μl of 1x SDS-PAGE loading buffer (50mM Tris-HCl pH 6.8, 2% SDS, 10% glycerol, 1% β-MeSH, 12.5mM EDTA, 0.02% bromophenol blue) supplemented with 1% Sigma Protease Inhibitor Cocktail (Cat. Num: P9599). Protein amounts in the extracts were determined by amidoblack. 80 μg (lane 1 and lane 2) or 20 μg (lane 3) of total protein was separated on 10 % SDS-PAGE and blotted for 1.5h to PVDF using semi-dry transfer. Blots were blocked with 4% Milk and 1% BSA in 1x TBST for 1h at room temperature (RT) with agitation. The blot was incubated in the primary antibody at a dilution of 1:1 000 in 1x TBST (0.1% Tween-20) with 1% BSA for 16h at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3x for 10 min in 1x TBST at RT with agitation. Blot was incubated in secondary antibody diluted to 1:5 000 in 1x TBST for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL (Advansta, K-12045-D20) according to the manufacturer's instructions. Exposure time was 120 seconds.

    The upper black arrow is mCherry-GRP-linker-WUS. It is a N-terminal fusion product of WUS, while the GFP is a C-terminal linked product (WUS-GFP).
    Courtesy of Matyas Medzihradszky, Centre for Organismal Studies, University of Heidelberg, Germany
  • Background
  • Background:

    WUSCHEL is a transcription factor which plays a central role during early embryogenesis, oogenesis, flowering. 

  • Product Citations
  • Selected references: Dory et al. (2016). Kinase-Associated Phosphoisoform Assay: a novel candidate-based method to detect specific kinase-substrate phosphorylation interactions in vivo. BMC Plant Biol. 2016 Sep 21;16(1):204.
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