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AGO set
Tag Antibody Set 

AtpH | ATP synthase subunit c (chloroplastic)

AS05 071  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, S. oleracea, N. benthamina, T. elongatus

AtpH | ATP synthase subunit c (chloroplastic) in the group Antibodies for Plant/Algal  / Photosynthesis  / ATP synthase at Agrisera AB (Antibodies for research) (AS05 071)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

Purified c subunit from Spinacia oleracea UniProt: P69447.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000-1 : 10 000 (WB)
Expected | apparent MW

8 kDa (for Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea, Thermosynechococcus elongatus
Predicted reactivity

Algae, Cannabis sativa, Glycine max, Oryza sativa, Physcomitrella patens, Pisum sativum, Populus alba, Pinus thunbergii, Zea mays, Vitis vinifera

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

AS05 071 western picture.jpg

0.7 μg of   purified ATP-synthase complex from Spinacia oleracea (CF0F1) (1), 15.3 µg of purified ATP-synthase complex (CF0F1) from Nicotiana benthamiana (2) and 48.6 µg of Thermosynechococcus elongatus thylakoid preparation (3) were separated on 12% polyacrylamide gel and blotted on PVDF membrane. Filters where blocked (0.5h), incubated with 1: 1000 anti-AtpH antibodies (1h), followed by incubation with 1: 5 000 secondary anti-rabbit antibody (1.25h), coupled to HRP and visualized with chemiluminescence detection reagent.

Additional information

Additional information

This product can be sold containing proclin if requested.

Note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur.

Related products

Related products

AS08 370 | Anti-ATP synthase whole enzyme, rabbit antibodies

AS08 304 | Anti-ATP synthase subunit alpha, rabbit antibodies

AS05 085 | Anti-ATP synthase subunit beta, rabbit antibodies

AS08 312 | Anti-ATP synthase subunit gamma, rabbit antibodies

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background

F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.

Product citations

Selected references Nath et al. (2016). A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins. Plant Physiol. 2016 Dec;172(4):2459-2470. Epub 2016 Oct 26.
Lawrence et al. (2010). Recombinant production and purification of the subunit c of chloroplast ATP synthase. Protein Expression and Purification 76: 15-24.

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