AtpH | ATP synthase subunit c (chloroplastic)
AS05 071 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, S. oleracea, N. benthamina, T. elongatus

Data sheet | Product citations | Protocols | Add review |
Product Information
Purified c subunit from Spinacia oleracea UniProt: P69447.
8 kDa (for Arabidopsis thaliana)
Reactivity
Algae, Cannabis sativa, Glycine max, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus alba, Pinus thunbergii, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Application examples
Application example

0.7 μg of purified ATP-synthase complex from Spinacia oleracea (CF0F1) (1), 15.3 µg of purified ATP-synthase complex (CF0F1) from Nicotiana benthamiana (2) and 48.6 µg of Thermosynechococcus elongatus thylakoid preparation (3) were separated on 12% polyacrylamide gel and blotted on PVDF membrane. Filters where blocked (0.5h), incubated with 1: 1000 anti-AtpH antibodies (1h), followed by incubation with 1: 5 000 secondary anti-rabbit antibody (1.25h), coupled to HRP and visualized with chemiluminescence detection reagent.
Additional information
This product can be sold containing proclin if requested.
Background
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.
Product citations
Lawrence et al. (2010). Recombinant production and purification of the subunit c of chloroplast ATP synthase. Protein Expression and Purification 76: 15-24.
Related products: AtpH | ATP synthase subunit c (chloroplastic)
This product can b...
10 % until...
10 ...