Beta-Galactosidase (mouse monoclonal) (clone BG-02)

Product no: AS21 4616
AS21 4616 | Clonality: Monoclonal | Host: Mouse | Reactivity: Escherichia coli
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  • Product Info
  • Immunogen: beta-Galactosidase purified from E. coli. UniProt: P00722
    Sub class: IgG1
    Host: Mouse
    Clonality: Monoclonal
    Purity: Immunoglobulin purified on Protein A.
    Format: Liquid at 1 mg/ml.
    Quantity: 0,1 mg
    Storage: Store at 2-8C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunocyto chemistry (ICC), Western blot (WB)
    Recommended dilution: 1 µg/ml (WB)
    Expected | apparent MW: 116,5 kDa
  • Reactivity
  • Confirmed reactivity: Escherichia coli, GalB-tagged fusion proteins
  • Application Examples
  • Western blot using anti- beta galactosidase from E.coli moncoclonal antibodies

    Purified beta galactosidase from E.coli, non-reduced sample was separated on SDS-PAGE and transferred to a membrane. Protein visualization was done using anti- beta galactosidase monoclonal antibodies at 1 µg/ml, followed by secondary anti-mouse IgG antibodies, HRP conjugated. Reaction was visualized using chemiluminescence, according to manufacture's recommendation.

  • Additional Information
  • Additional information: This antibody recognizes a non-catalytic centre epitope of beta-galactosidase from E. coli.
  • Background
  • Background: Beta-Galactosidase is an enzyme (EC:3.2.1.23) involved in hydrolysis of terminal non-reducing beta-D-galactose residues into beta-D-galactosides. The protein is encoded by lacZ gene.

    Alternative names: Beta-gal, Lactase, GalB
  • Protocols
  • Antibody protocols
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