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Beta-Galactosidase (mouse monoclonal) (clone BG-02)

AS21 4616 | Clonality: Monoclonal | Host: Mouse | Reactivity: Escherichia coli
Beta-Galactosidase (mouse monoclonal) (clone BG-02) in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS21 4616)

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Product Information

Immunogen beta-Galactosidase purified from E. coli. UniProt: P00722
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Purified by protein A affinity chromatography
Format Liquid at 1 mg/ml
Quantity 0,1 mg
Storage Store at 2-8°C, Do not freeze, Do not use after expiration date stamped on the label, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes
Tested applications Immunocyto chemistry (ICC), Western blot (WB)
Recommended dilution 1 µg/ml (WB)
Expected | apparent MW 116,5 kDa

Reactivity

Confirmed reactivity Escherichia coli, GalB-tagged fusion proteins

Application examples

Application examples Western blot using anti- beta galactosidase from E.coli moncoclonal antibodies

Purified beta galactosidase from E.coli, non-reduced sample was separated on SDS-PAGE and transferred to a membrane. Protein visualization was done using anti- beta galactosidase monoclonal antibodies at 1 µg/ml, followed by secondary anti-mouse IgG antibodies, HRP conjugated. Reaction was visualized using chemiluminescence, according to manufacture's recommendation.

Additional information

Additional information This antibody recognizes a non-catalytic centre epitope of beta-galactosidase from E. coli.

Related products

Background

Background Beta-Galactosidase is an enzyme (EC:3.2.1.23) involved in hydrolysis of terminal non-reducing beta-D-galactose residues into beta-D-galactosides. The protein is encoded by lacZ gene.

Alternative names: Beta-gal, Lactase, GalB

Product citations

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