BZR1 | Brassinazole resistant 1 (Oryza sativa)

AS16 3219 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Oryza sativa

Limited stock.

BZR1 | Brassinazole resistant 1 (Oryza sativa)

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Oryza sativa BZR1 protein, UniProt: Q7XI96

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage The antibody may be stored at -20°C for one year in its original formulation. Additionally, antibody may be stored at 2°C to 8°C for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.

Tested applications Western blot (WB)

Recommended dilution 1 : 500-1 : 1000 (WB)

Expected | apparent MW

31 kDa

Reactivity

Confirmed reactivity Oryza sativa

Predicted reactivity Hordeum vulgare, Zea mays
Species of your interest not listed? Contact us

Not reactive in Arabidopsis thaliana

Application examples

Application examples application example

Total protein from Oryza sativa rice (CV. 9311) flag leaf at the tillering stage was ground into a fine powder in liquid nitrogen. An 800 ul aliquot of extraction buffer [62.5 mM TRIS-HCl (pH 7.4), 10% glycerol, 0.1% SDS, 2 mM EDTA, 1 mM phenylmethylsulphonyl fluoride (PMSF), 5% (v/v) b-mercaptoethanol] was added to each 300 mg powder sample. The mixture was vortexed and then chilled on ice for 10 min. Samples were centrifuged at 12 000 rpm for 10 min at 4℃, and the supernatant was collected and stored at –70 ℃. The protein concentrations of the rice samples were determined using the Bradford method (Bradford, 1976). 20 µg of protein was separated on 12 % SDS-PAGE and blotted 1h to PVDF.
Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 2 min

Additional information

Background

Background The brassinosteroid (BR) signaling pathway is regulated by complex mechanisms which at the transcriptional level involves BRI1 negatively regulated by BZR1 and BES1. BZR1 mediates downstream brassinosteroid-regulated growth response and feedback inhibition of brassinosteroid biosynthetic genes.

Product citations

Picture (footer):	application example Total protein from Oryza sativa rice (CV. 9311) flag leaf at the tillering stage was ground into a fine powder in liquid nitrogen. An 800 ul aliquot of extraction buffer [62.5 mM TRIS-HCl (pH 7.4), 10% glycerol, 0.1% SDS, 2 mM EDTA, 1 mM phenylmethylsulphonyl fluoride (PMSF), 5% (v/v) b-mercaptoethanol] was added to each 300 mg powder sample. The mixture was vortexed and then chilled on ice for 10 min. Samples were centrifuged at 12 000 rpm for 10 min at 4℃, and the supernatant was collected and stored at –70 ℃. The protein concentrations of the rice samples were determined using the Bradford method (Bradford, 1976). 20 µg of protein was separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 2 min
Confirmed reactivity:	Oryza sativa
predicted reactivity:	Hordeum vulgare, Zea mays Species of your interest not listed? Contact us
not reactive in:	Arabidopsis thaliana
calculated \| apparent molecular mass [kDa]:	31 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide derived from Oryza sativa BZR1 protein, UniProt: Q7XI96
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 500-1 : 1000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	The antibody may be stored at -20°C for one year in its original formulation. Additionally, antibody may be stored at 2°C to 8°C for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.
tested applications:	Western blot (WB)
background:	The brassinosteroid (BR) signaling pathway is regulated by complex mechanisms which at the transcriptional level involves BRI1 negatively regulated by BZR1 and BES1. BZR1 mediates downstream brassinosteroid-regulated growth response and feedback inhibition of brassinosteroid biosynthetic genes.