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BZR1 | Brassinazole resistant 1 (Oryza sativa)

AS16 3219 | Clonality: Polyclonal | Host: Rabbit | Reactivity:

BZR1 | Brassinazole resistant 1 (Oryza sativa) in the group Antibodies for Plant/Algal  / Hormones / Brassinosteroids/regulation at Agrisera AB (Antibodies for research) (AS16 3219)

DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Oryza sativa BZR1 protein, UniProt: Q7XI96

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage The antibody may be stored at -20℃for one year in its original formulation. Additionally, antibody may be stored at 2℃ to 8℃ for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution 1 : 500-1 : 1000 (WB)
Expected | apparent MW

31 kDa

Reactivity

Confirmed reactivity
Predicted reactivity Hordeum vulgare
Species of your interest not listed? Contact us
Not reactive in Arabidopsis thaliana

Application examples

Application examples application example

western blot using anti-BZR1 polyclonal antibodies



Total protein from Oryza sativa rice (CV. 9311) flag leaf at the tillering stage was ground into a fine powder in liquid nitrogen. An 800 ul aliquot of extraction buffer [62.5 mM TRIS-HCl (pH 7.4), 10% glycerol, 0.1% SDS, 2 mM EDTA, 1 mM phenylmethylsulphonyl fluoride (PMSF), 5% (v/v) b-mercaptoethanol] was added to each 300 mg powder sample. The mixture was vortexed and then chilled on ice for 10 min. Samples were centrifuged at 12 000 rpm for 10 min at 4℃, and the supernatant was collected and stored at –70 ℃. The protein concentrations of the rice samples were determined using the Bradford method (Bradford, 1976). 20 µg of protein was separated on 12 % SDS-PAGE and blotted 1h to PVDF.
Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 2 min

Additional information

Related products

Related products

AS16 3203 | Anti-BIN2 | BRASSINOSTEROID INSENSITIVE 2, rabbit antibodies

Secondary antibodies

Background

Background The brassinosteroid (BR) signaling pathway is regulated by complex mechanisms which at the transcriptional level involves BRI1 negatively regulated by BZR1 and BES1. BZR1 mediates downstream brassinosteroid-regulated growth response and feedback inhibition of brassinosteroid biosynthetic genes.

Product citations

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