Anti-DOG1 alpha splice variant

Product no: AS15 3032

AS15 3032 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Replaced by AS24 5008. Interested to receive a free sample for testing? Contact us.

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  • Product Info
  • Immunogen:

    Synthetic peptide, conjugated to KLH derived from a sequence of Arabidopsis thaliana DOG1, UniProt:A0SVK0, TAIR: AT5G45830

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000 (WB)
    Expected | apparent MW: 32.4 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Brachypodium distachyon, Brassica napus, Capsella rubella, Lepidium papillosum, Sisymbrium officinale

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Background
  • Background:

    DOG1 alpha splice variant (Delay Of Germination 1) is involved in the control of seed dormancy.

    Alternative names: ATDOG1, DELAY OF GERMINATION 1, DOG1, GAAS5, GERMINATION ABILITY AFTER STORAGE 5, GLUCOSE SENSING QTL 5, GSQ5.

  • Product Citations
  • Selected references: Bryant et al. (2019). Basic LEUCINE ZIPPER TRANSCRIPTION FACTOR67 Transactivates DELAY OF GERMINATION1 to Establish Primary Seed Dormancy in Arabidopsis. Plant Cell. 2019 Jun;31(6):1276-1288. doi: 10.1105/tpc.18.00892
    Cyrek et al. (2016). Seed Dormancy in Arabidopsis Is Controlled by Alternative Polyadenylation of DOG1. Plant Physiol. 2016 Feb;170(2):947-55. doi: 10.1104/pp.15.01483.
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
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    Number of reviews: (1)
    Anna Kulik | 2023-12-28
    0 | 0
    Antibodies work nicely for Western Blot in seeds. We were able to easly detect DOG1 protein when loading 35 ug of total protein extract per sample. No band was detected in dog1 KO mutants.

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