GFP | Green Fluorescence Protein, clone 1A5 (rat monoclonal)

AS20 4511 | Clonality: Monoclonal | Host: Rat | Reactivity: GFP and EGFP

Product Information

Immunogen

Recombinant GFP protein from Aequorea victoria, UniProt: P42212

Host Rat

Clonality Monoclonal

Subclass/isotype IgG1

Purity Immunoglobulin Protein A purified in PBS, 50 % glycerol, filter sterilized.

Format Liquid, 1mg/ml.

Quantity 100 µg

Storage Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Chromatin immunoprecipitation (ChIP), ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1 : 5000-1 : 25 000 (ELISA), 1 : 500 (IF), 1 : 2000-1 : 10 000 (WB)

Reactivity

Confirmed reactivity Native GFP, Recombinant GFP (E,coli), all variants of GFP, including EGFP

Application examples

Application examples Western blot using anti-GFP rat monoclonal antibodies

Detection of GFP-ZIPK fusion protein over-expressed in HEK293 cells by Western protein with antibody 1A5. (-) HEK293T cell extract without overexpression (+) GFP-ZIPK protein- overexpressed HEK293T cell extract

Western blot using anti-GFP rat monoclonal antibodies

Immunoprecipitation of GST-GFP fusion blotting with antibody 1A5.

Fluorescent image of COS1 cells with GFP of GST-ZIPK fusion protein expressed in HEK293T cells (Right) and the same cells were immunostained by using anti-GFP antibody 1A5, followed by Texas Red-conjugated anti-rat IgG (Left). Note that fluorescence by the immunofluorescent staining using 1A5 antibody is much stronger than fluorescence due to GFP (right panel).

Additional information

Additional information Antibody detects GFP and EGFP

Background

GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

Product citations

Selected references Maehara et al. (2015). issue-specific expression of histone H3 variants diversified after species separation. Epigenetics Chromatin. 2015 Sep 17;8:35. doi: 10.1186/s13072-015-0027-3. (ChIP)
Okazaki et al. (2012). Nuclear localization signal in a cancer-related transcriptional regulator protein NAC1. Carcinogenesis. 2012 Oct;33(10):1854-62.doi: 10.1093/carcin/bgs193. (Immunoprecipiation)

Picture (footer):	Detection of GFP-ZIPK fusion protein over-expressed in HEK293 cells by Western protein with antibody 1A5. (-) HEK293T cell extract without overexpression (+) GFP-ZIPK protein- overexpressed HEK293T cell extract Immunoprecipitation of GST-GFP fusion blotting with antibody 1A5. Fluorescent image of COS1 cells with GFP of GST-ZIPK fusion protein expressed in HEK293T cells (Right) and the same cells were immunostained by using anti-GFP antibody 1A5, followed by Texas Red-conjugated anti-rat IgG (Left). Note that fluorescence by the immunofluorescent staining using 1A5 antibody is much stronger than fluorescence due to GFP (right panel).
All references:	Maehara et al. (2015). issue-specific expression of histone H3 variants diversified after species separation. Epigenetics Chromatin. 2015 Sep 17;8:35. doi: 10.1186/s13072-015-0027-3. (ChIP) Okazaki et al. (2012). Nuclear localization signal in a cancer-related transcriptional regulator protein NAC1. Carcinogenesis. 2012 Oct;33(10):1854-62.doi: 10.1093/carcin/bgs193. (Immunoprecipiation)
additional information:	Antibody detects GFP and EGFP
background:	GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Confirmed reactivity:	Native GFP, Recombinant GFP (E,coli), all variants of GFP, including EGFP
Clonality:	Monoclonal
Format:	Liquid, 1mg/ml.
Host:	Rat
immunogen:	Recombinant GFP protein from Aequorea victoria, UniProt: P42212
Purity:	Immunoglobulin Protein A purified in PBS, 50 % glycerol, filter sterilized.
Quantity:	100 µg
recommended dilution:	1 : 5000-1 : 25 000 (ELISA), 1 : 500 (IF), 1 : 2000-1 : 10 000 (WB)
storage:	Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Sub class:	IgG1
tested applications:	Chromatin immunoprecipitation (ChIP), ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)

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