GID2 | F-BOX protein GID2 (SLEEPY 1)

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AS13 2638 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


48 st
Item No:
AS13 2638

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product information

GID2 | F-BOX protein GID2 (SLEEPY 1) is an essential component of the SCF-type E3 ligase complex, which positively regulates the gibberellin signaling pathway. It mediates the ubiquitination and subsequent degradation of DELLA proteins (GAI, RGA and RGL2). Localized in nucleus and expressed in all tissues tested so far.  


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana GID2 sequence, UniProt: Q9STX3, TAIR: At4g24210

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS14 2800 | GID1c | Gibberellin receptor GID1C, rabbit antibody
AS11 1631 | GAI | DELLA protein GAI, rabbit antibody
AS11 1630 | RGA | DELLA protein RGA, rabbit antibody
AS11 1803 | RGA-like protein 2 | DELLA protein RGL2, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

17.4 | 18 kDa (Arabidopsis)

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica napus, Medicago truncatula, Glycine max, Ricinus communis, Populus trichocarpa, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

to be added when available, antibody released in April 2014.

application example

western blot using anti-GID2 antibodies

15µg of total protein from inflorescence of Col0, spy mutant, and 35S-SPY extracted with extraction buffer (DTT 100uM, Tris pH 6,8 67,5mM,  Urea 4M, SDS 3%,glycerol 3% and bromophenol 0,1%) were separated on  10% SDS-PAGE and blotted 1h to PVDF. Blots were blocked with milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 overnight at 4°C with  agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with  agitation. Blot was incubated in secondary antibody diluted to  1:10 000 in TBS-T for 1h at RT with agitation. The blot was washed as  above and developed for 2 min with ECL (Luminata Forte) according to  the manufacturer's instructions. Exposure time was 30 seconds.

Courtesy Dr. Patrick Achard, CNRS, France

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