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GID2 | F-BOX protein GID2 (SLEEPY 1)

AS13 2638 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

GID2 | F-BOX protein GID2 (SLEEPY 1)  in the group Antibodies for Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS13 2638)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana GID2 sequence, UniProt: Q9STX3, TAIR: At4g24210

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

17.4 | 18 kDa (Arabidopsis)

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica napus, Medicago truncatula, Glycine max, Ricinus communis, Populus trichocarpa, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-GID2 antibodies

15µg of total protein from inflorescence of Col0, spy mutant, and 35S-SPY extracted with extraction buffer (DTT 100uM, Tris pH 6,8 67,5mM,  Urea 4M, SDS 3%,glycerol 3% and bromophenol 0,1%) were separated on  10% SDS-PAGE and blotted 1h to PVDF. Blots were blocked with milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 overnight at 4°C with  agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with  agitation. Blot was incubated in secondary antibody diluted to  1:10 000 in TBS-T for 1h at RT with agitation. The blot was washed as  above and developed for 2 min with chemiluminescent detection reagent, according to  the manufacturer's instructions. Exposure time was 30 seconds.

Courtesy Dr. Patrick Achard, CNRS, France

Additional information

Related products

Related products

AS14 2800 | Anti-GID1c | Gibberellin receptor GID1C, rabbit antibodies
AS11 1631 | Anti-GAI | DELLA protein GAI, rabbit antibodies
AS11 1630 | Anti-RGA | DELLA protein RGA, rabbit antibodies
AS11 1803 | Anti-RGA-like protein 2 | DELLA protein RGL2, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

GID2 | F-BOX protein GID2 (SLEEPY 1) is an essential component of the SCF-type E3 ligase complex, which positively regulates the gibberellin signaling pathway. It mediates the ubiquitination and subsequent degradation of DELLA proteins (GAI, RGA and RGL2). Localized in nucleus and expressed in all tissues tested so far.  

Product citations

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