GS | Glutathione synthase, GSH-S

Name: GS | Glutathione synthase, GSH-S
SKU: AS06 183
Price: 394 EUR
Availability: InStock

AS06 183 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Brassica campestris, Nicotiana tabacum, Phaeodactylum tricornutum, Solanum lycopersicum, Zea mays

GS | Glutathione synthase, GSH-S in the group Antibodies Plant/Algal / Environmental Stress / Plant Oxidative Stress at Agrisera AB (Antibodies for research) (AS06 183)

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Zea mays GSH-S sequence Q8W4W2

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein G purified in PBS pH 7.4.

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunolocalization (IL), Western blot (WB)

Recommended dilution 1 : 2000 (WB)

Expected | apparent MW

45 kDa

Reactivity

Confirmed reactivity Brassica campestris, Nicotiana tabacum, Oryza sativa, Phaeodactylum tricornutum, Solanum lycopersicum, Zea mays

Predicted reactivity Aegilops tauschii, Arabidopsis thaliana, Arundo donax, Asparagus officinalis, Brachypodium distachyon, Capsicum annuum, Cicer arietinum, Citrus clementina, Corchorus olitorius, Cucumis sativus, Daucus carota subsp. sativus, Dimocarpus longan, Eucalyptus grandis, Gossypium hirsutum , Helianthus annuus , Hevea brasiliensis, Hordeum vulgare, Jatropha curcas, Lotus japonicus, Lupinus angustifolius, Lycium chinense, Musa acuminata, Nelumbo nucifera, Nicotiana tabacum, Nicotiana sylvestris, Pisum sativum, Setaria italica, Sorghum bicolor, Spinacia oleracea, Triticum aestivum, Zostera marina, Vitis vinifera

Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Total IgG concentration is 3,7 µg/µl

Immunolocalization has been done for Arabidopsis thaliana and Nicotiana tabacum

Background

Glutathione synthetase (GSH-S) catalyses the last step of glutathione (GSH) synthesis. GSH is synthesized in two ATP-dependent steps: in the first, gamma-glutamylcysteine (gamma-EC) is synthesized from L-glutamate and cysteine, catalyzed by gamma-glutamylcysteine synthetase (g-ECS); in the second step, catalysed by GSH-S, glycine is added to the C-terminal end of gamma-EC. GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development

Product citations

Selected references Sun et a. (2019). Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots of Brassica campestris L. in Response to Cadmium Stress. Int J Mol Sci. 2019 Dec 26;21(1). pii: E180. doi: 10.3390/ijms21010180.
Jayawardena et al. (2016). Elevated CO2 plus chronic warming reduces nitrogen uptake and levels or activities of nitrogen -uptake and -assimilatory proteins in tomato roots. Physiol Plant. 2016 Nov 28. doi: 10.1111/ppl.12532. [Epub ahead of print]
Baojian et al. (2014). Maize (Zea mays L.) seedling leaf nuclear proteome and differentially expressed proteins between a hybrid and its parental lines. Proteomics, DOI: 10.1002/pmic.201300147.
Gomez et al. (2004) Intercellular distribution of glutathione synthesis in maize leaves and its response to short term chilling. Plant Physiol. 134: 1662-1671.

immunogen:	KLH-conjugated synthetic peptide derived from Zea mays GSH-S sequence Q8W4W2
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Total IgG. Protein G purified in PBS pH 7.4.
Format:	Lyophilized
Quantity:	100 µl
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Immunolocalization (IL), Western blot (WB)
recommended dilution:	1 : 2000 (WB)
Expected \| apparent MW:	45 kDa
Confirmed reactivity:	Brassica campestris, Nicotiana tabacum, Oryza sativa, Phaeodactylum tricornutum, Solanum lycopersicum, Zea mays
predicted reactivity:	Aegilops tauschii, Arabidopsis thaliana, Arundo donax, Asparagus officinalis, Brachypodium distachyon, Capsicum annuum, Cicer arietinum, Citrus clementina, Corchorus olitorius, Cucumis sativus, Daucus carota subsp. sativus, Dimocarpus longan, Eucalyptus grandis, Gossypium hirsutum , Helianthus annuus , Hevea brasiliensis, Hordeum vulgare, Jatropha curcas, Lotus japonicus, Lupinus angustifolius, Lycium chinense, Musa acuminata, Nelumbo nucifera, Nicotiana tabacum, Nicotiana sylvestris, Pisum sativum, Setaria italica, Sorghum bicolor, Spinacia oleracea, Triticum aestivum, Zostera marina, Vitis vinifera Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Total IgG concentration is 3,7 µg/µl
additional information (application):	Immunolocalization has been done for Arabidopsis thaliana and Nicotiana tabacum
background:	Glutathione synthetase (GSH-S) catalyses the last step of glutathione (GSH) synthesis. GSH is synthesized in two ATP-dependent steps: in the first, gamma-glutamylcysteine (gamma-EC) is synthesized from L-glutamate and cysteine, catalyzed by gamma-glutamylcysteine synthetase (g-ECS); in the second step, catalysed by GSH-S, glycine is added to the C-terminal end of gamma-EC. GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development
All references:	Sun et a. (2019). Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots of Brassica campestris L. in Response to Cadmium Stress. Int J Mol Sci. 2019 Dec 26;21(1). pii: E180. doi: 10.3390/ijms21010180. Jayawardena et al. (2016). Elevated CO2 plus chronic warming reduces nitrogen uptake and levels or activities of nitrogen -uptake and -assimilatory proteins in tomato roots. Physiol Plant. 2016 Nov 28. doi: 10.1111/ppl.12532. [Epub ahead of print] Baojian et al. (2014). Maize (Zea mays L.) seedling leaf nuclear proteome and differentially expressed proteins between a hybrid and its parental lines. Proteomics, DOI: 10.1002/pmic.201300147. Gomez et al. (2004) Intercellular distribution of glutathione synthesis in maize leaves and its response to short term chilling. Plant Physiol. 134: 1662-1671.