showForm Anti-GTA MCP | Gene Transfer Agent (GTA) major capsid protein (MCP) antibodies

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GTA MCP | Gene Transfer Agent (GTA) major capsid protein (MCP)

AS08 365  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Rhodobacterales

GTA MCP | Gene Transfer Agent (GTA) major capsid protein (MCP) in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS08 365)

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Product Information

Immunogen

KLH-conjugated conserved peptide sequence found in the Gene Transfer Agent (GTA) major capsid protein (MCP) encoded in Bacteria within the Rhodobacterales order of the class alpha-Proteobacteria including Rhodobacter sphaeroides UniProt: Q3J3K4

 

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile distilled water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

31.4 | 32 kDa (predicted mature capsid protein of Rhodobacter capsulatus)

Reactivity

Confirmed reactivity Dinoroseobacter shibae, Rhodobacter capsulatus, Ruegeria pomeroyi DSS-3
Predicted reactivity

Rhodobacterales

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

 

western blot detection using anti-GTA antibodies

Roseobacter capsulatus cells, pelleted by centrifugation and resuspended in an equial volume of TE buffer and supernatant sample* were separated on  10% SDS-PAGE and blotted 1h to nitrocellulose. The "+" indicated R. capsulatus SB1003 (GTA positive) and "-" indicated R. capsulatus A1 (GTA capsid protein negative). Blots were blocked in 5% skim milk in TBST followed by incubation with anti-GTA antibodies (AS08 365) at dilution 1: 1000 at 4°C over night. After washes blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Santa Cruz Biotechnology, Santa Cruz, CA) and specific bands were detected with chemiluminescence detection reagent.Exposure time was 30 seconds with CCD camera.

* - supernatant sample was obtained in a following way: cells were removed by two rounds of centrifugation at 17,000 g for 2 min. with sub-sample of the supernatant removed to a new tube. Two volumes of the cells or final culture supernatant were mixed with 1 volume of  3X SDS-PAGE sample buuffer (NEB).

Additional information

Additional information This product can be sold containing ProClin if requested.

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Background

Background

Gene Transfer Agents (GTAs) are bacteriophage-like particles which function as a means to package and transfer genomic DNA between bacterial cells.GTA-mediated gene transfer might be an important phenomenon in natural microbial communities.

Product citations

Selected references Koppenhöfer et al. (2019). Integrated Transcriptional Regulatory Network of Quorum Sensing, Replication Control, and SOS Response in Dinoroseobacter shibae. Front. Microbiol., 12 April 2019 | https://doi.org/10.3389/fmicb.2019.00803.
Tomasch et al. (2018). Packaging of Dinoroseobacter shibae DNA into Gene Transfer Agent Particles Is Not Random. Genome Biol Evol. 2018 Jan 1;10(1):359-369. doi: 10.1093/gbe/evy005.
Mercer and Lang (2014). Identification of a predicted partner-switching system that affects production of the gene transfer agent RcGTA and stationary phase viability in Rhodobacter capsulatus. BMC Microbiol. 2014 Mar 19;14(1):71.

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