HSP70 | salmonid heat shock protein 70

Product no: AS05 061

AS05 061 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Salmo sp. (salmon), Salvelinus fontinalis (brook trout)

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 UniProt: B5X4Z3.

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 l
    Reconstitution: For reconstitution add 100 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 5 000 (WB)
    Expected | apparent MW:

    70 kDa

  • Reactivity
  • Confirmed reactivity: Rainbow trout (Oncorhynchus mykiss), Brook trout gills (Salvelinus fontinalis)
    Predicted reactivity:

    Salmo salar (Atlantic salmon)

    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: The antibody is very specific for salmonid inducible form - hsp70, It does not cross-react with hsc70, It does not detect hsp70 from other species
  • Background
  • Background:

    Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.

  • Product Citations
  • Selected references: Biela et al. (2020). Evidence of prevalent heat stress in Yukon River Chinook salmon. Canadian J. of Fisheriers and Aquatic Science.
    Kelly et al. (2017). Acclimation capacity of the cardiac HSP70 and HSP90 response to thermal stress in lake trout (Salvelinus namaycush), a stenothermal ice-age relict. Comp Biochem Physiol B Biochem Mol Biol. 2017 Dec 10. pii: S1096-4959(17)30191-4. doi: 10.1016/j.cbpb.2017.12.002.
    Ricketts et al. (2015). The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss. PLoS One. 2015 Jan 28;10(1):e0115334. doi: 10.1371/journal.pone.0115334. eCollection 2015.
    Templeman et al. (2014). Linking physiological and cellular responses to thermal stress: -adrenergic blockade reduces the heat shock response in fish. J Comp Physiol B, April 2014.
  • Protocols
  • Antibody protocols
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