LEA6 | Late embryogenesis abundant protein 6

Product no: AS21 4596

AS21 4596  | Clonality:  Polyclonal |  Host:  Rabbit | Reactivity: Arabidopsis thaliana (recombinant LEA6)

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana LEA6 protein sequences, UniProt: O64820, TAIR: At2g23110, UniProt: Q8S8R1 TAIR: AT2G23120 and UniProt: O23658, TAIR: AT2G33690




    Purity: Affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana (recombinant LEA6)
    Predicted reactivity: Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot detection using anti-LEA6 antibodies
    M - Precision plus protein dual colour standard Bio-Rad
    1 - Bacterial extract, 4 µl
    2 - Purified, His-tagged Arabidopsis thaliana LEA 16 protein, 8 µl

    Samples were denatured with SDS DTT sample buffer (12 mM Tris pH 6.8, 10 % glycerol, 0.4 % SDS, 80 mM DTT, 0.02 % bromophenol blue) for 30 min at room temperature, then were separated on 12 % SDS-PAGE and blotted 1h at 100 V to PVDF membrane (pore size of 0.2 µm), using wet transfer (10 mM CAPS pH 11, 20% MeOH). Blot was blocked with 5% milk in TBS 0.1 % Tween20 for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight/4°C in TBS 0.1 % Tween 20 without agitation. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed 2 times for 30 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in 25 mL of TBS 0.1 %Tween20 for 1h/RT with agitation. The blot was washed as above and developed for 5 min with Bio-Rad Clarity western ECL substrate. Exposure time was 60 seconds.

    Note: The apparent molecular mass (32-34 kDa) is 4 times higher than expected (8 kDa). This is consistent with publications that reported the aberrant behavior of some LEA proteins in SDS-PAGE.

    Courtesy of Dr. David Macherel, IRHS/Seedling Metabolism & Stress, France

  • Additional Information
  • Additional information (application): Antibody reactivity in endogenous sample remains to be determined.
  • Background
  • Background: LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress.

    Synonymes: Putative late embryogenesis abundant protein LEA.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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