LEA6 | Late embryogenesis abundant protein 6

AS21 4596 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana (recombinant LEA6)

Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana LEA6 protein sequences, UniProt: O64820, TAIR: At2g23110, UniProt: Q8S8R1 TAIR: AT2G23120 and UniProt: O23658, TAIR: AT2G33690

Host

Rabbit

Clonality

Polyclonal

Purity Affinity purified serum, in PBS pH 7.4

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl, of sterile or deionized water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Reactivity

Confirmed reactivity Arabidopsis thaliana (recombinant LEA6)

Predicted reactivity Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot detection using anti-LEA6 antibodies

Samples:
M - Precision plus protein dual colour standard Bio-Rad
1 - Bacterial extract, 4 µl
2 - Purified, His-tagged Arabidopsis thaliana LEA 16 protein, 8 µl

Samples were denatured with SDS DTT sample buffer (12 mM Tris pH 6.8, 10 % glycerol, 0.4 % SDS, 80 mM DTT, 0.02 % bromophenol blue) for 30 min at room temperature, then were separated on 12 % SDS-PAGE and blotted 1h at 100 V to PVDF membrane (pore size of 0.2 µm), using wet transfer (10 mM CAPS pH 11, 20% MeOH). Blot was blocked with 5% milk in TBS 0.1 % Tween20 for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight/4°C in TBS 0.1 % Tween 20 without agitation. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed 2 times for 30 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in 25 mL of TBS 0.1 %Tween20 for 1h/RT with agitation. The blot was washed as above and developed for 5 min with Bio-Rad Clarity western ECL substrate. Exposure time was 60 seconds.

Note: The apparent molecular mass (32-34 kDa) is 4 times higher than expected (8 kDa). This is consistent with publications that reported the aberrant behavior of some LEA proteins in SDS-PAGE.

Courtesy of Dr. David Macherel, IRHS/Seedling Metabolism & Stress, France

Additional information

Antibody reactivity in endogenous sample remains to be determined.

Background

Background LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress.

Synonymes: Putative late embryogenesis abundant protein LEA.

Product citations

Picture (footer):	Samples: M - Precision plus protein dual colour standard Bio-Rad 1 - Bacterial extract, 4 µl 2 - Purified, His-tagged Arabidopsis thaliana LEA 16 protein, 8 µl Samples were denatured with SDS DTT sample buffer (12 mM Tris pH 6.8, 10 % glycerol, 0.4 % SDS, 80 mM DTT, 0.02 % bromophenol blue) for 30 min at room temperature, then were separated on 12 % SDS-PAGE and blotted 1h at 100 V to PVDF membrane (pore size of 0.2 µm), using wet transfer (10 mM CAPS pH 11, 20% MeOH). Blot was blocked with 5% milk in TBS 0.1 % Tween20 for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight/4°C in TBS 0.1 % Tween 20 without agitation. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed 2 times for 30 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in 25 mL of TBS 0.1 %Tween20 for 1h/RT with agitation. The blot was washed as above and developed for 5 min with Bio-Rad Clarity western ECL substrate. Exposure time was 60 seconds. Note: The apparent molecular mass (32-34 kDa) is 4 times higher than expected (8 kDa). This is consistent with publications that reported the aberrant behavior of some LEA proteins in SDS-PAGE. Courtesy of Dr. David Macherel, IRHS/Seedling Metabolism & Stress, France
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated peptide derived from Arabidopsis thaliana LEA6 protein sequences, UniProt: O64820, TAIR: At2g23110, UniProt: Q8S8R1 TAIR: AT2G23120 and UniProt: O23658, TAIR: AT2G33690
Purity:	Affinity purified serum, in PBS pH 7.4
Quantity:	50 µg
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 50 µl, of sterile or deionized water.
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications:	Western blot (WB)
additional information (application):	Antibody reactivity in endogenous sample remains to be determined.
Confirmed reactivity:	Arabidopsis thaliana (recombinant LEA6)
predicted reactivity:	Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
background:	LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress. Synonymes: Putative late embryogenesis abundant protein LEA.