Lhcb5 | CP26 (Lhcb5) homolog (Ostreococcus tauri)

AS09 513 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Coccomyxa subellipsoidea, Ostreococcus tauri

Lhcb5 | CP26 (Lhcb5) homolog (Ostreococcus tauri)

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Lhcb4 (CP29) protein sequence from Ostreococcus taurii Q3B9U2

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 2000 (WB)

Expected | apparent MW

33 kDa

Reactivity

Confirmed reactivity Coccomyxa subellipsoidea, Ostreococcus tauri

Predicted reactivity

Algae, Ostreococcus lucimarinus

Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

Western blot using anti-Lhcb5 | CP26 (Lhcb5) homolog (Ostreococcus tauri) antibodies

1.0-4.5 µg of chlorophyll from Coccomyxa subellipsoidea cells were loaded to lanes. Samples were denatured with Laemmli buffer at 75°C for 5 min and were separated on 12% SDS-PAGE and blotted 30 min to PVDF using wet transfer. Blot was blocked with 5% milk in TBS for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 overnight at 4°C with agitation in 1% milk in TBS-T. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 1% milk in TBS-T for 1h at RT with agitation. The blot was washed 5 times for 5 min in TBS-T and 2 times for 5 min in TBS, and developed for 1 min with 1.25 mM luminol, 0.198 mM coumaric acid and 0.009% H₂O₂ in 0.1 M Tris- HCl, pH 8.5. Exposure time in ChemiDoc System was 115 seconds.

Courtesy MSc Paweł Rogowski, Warsaw University, Poland

Additional information

Background

Lhcb5 is one of the 3 minor, highly conserved chlorophyll a/b-binding proteins associated with Photosystem II in plants and algae. As a part of the inner light-harvesting antenna it has been sugested to regulate (together with Lhcb4 and Lhcb6) the energy flow from the outer LHCII antenna to the PSII reaction center.

Product citations

background:	Lhcb5 is one of the 3 minor, highly conserved chlorophyll a/b-binding proteins associated with Photosystem II in plants and algae. As a part of the inner light-harvesting antenna it has been sugested to regulate (together with Lhcb4 and Lhcb6) the energy flow from the outer LHCII antenna to the PSII reaction center.
Confirmed reactivity:	Coccomyxa subellipsoidea, Ostreococcus tauri
predicted reactivity:	Algae, Ostreococcus lucimarinus Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Application example 1.0-4.5 µg of chlorophyll from Coccomyxa subellipsoidea cells were loaded to lanes. Samples were denatured with Laemmli buffer at 75°C for 5 min and were separated on 12% SDS-PAGE and blotted 30 min to PVDF using wet transfer. Blot was blocked with 5% milk in TBS for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 overnight at 4°C with agitation in 1% milk in TBS-T. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 1% milk in TBS-T for 1h at RT with agitation. The blot was washed 5 times for 5 min in TBS-T and 2 times for 5 min in TBS, and developed for 1 min with 1.25 mM luminol, 0.198 mM coumaric acid and 0.009% H₂O₂ in 0.1 M Tris- HCl, pH 8.5. Exposure time in ChemiDoc System was 115 seconds. Courtesy MSc Paweł Rogowski, Warsaw University, Poland
calculated \| apparent molecular mass [kDa]:	33 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide derived from Lhcb4 (CP29) protein sequence from Ostreococcus taurii Q3B9U2
Purity:	Serum
Quantity:	200 µl
recommended dilution:	1 : 2000 (WB)
Reconstitution:	For reconstitution add 200 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)

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