LHCSR1

AS14 2819 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Bryopsis corticulans, Chlamydomonas reinhardtii, Nannochloropsis gaditana

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from LHCSR1 protein sequence from Chlamydomonas reinhardtii, UniProt: P93664

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

27 kDa

Reactivity

Confirmed reactivity Bryopsis corticulans, Chlamydomonas reinhardtii, Nannochloropsis gaditana

Predicted reactivity
Species of your interest not listed? Contact us

Not reactive in Lobosphaera incisa, Phaeodactylum tricornutum

Application examples

Application example

10 ug of a total cell extract of Chlamydomonas reinhardtii: WT _P _HL_High CO2 (1), WT_P_HL (2), Npq4_P _HL (3), were separated on Bolt® 4-12% Bis-Tris Plus Gels (precast) and blotted to PVDF using Bolt® transfer system for 1h . Blots were blocked with 5% BSA/milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and

3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera) diluted to 1:10 000 in for 1h at RT with

agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10 seconds.

P: photoautotrophically grown cells

HL: High light illumination(light intensity:500 μE)

Courtesy Dr. Roberta Croce, Biophysics of Photosynthesis Dep. Physics and Astronomy Faculty of Sciences VU University Amsterdam, The Netherlands

Additional information

This antibody is also recognizing recombinant LHCSR1 overexpressed in E.coli as described in Perozeni et al. (2020).

Background

LHCSR1 (Stress-related chlorophyll a/b binding protein 1) plays a role in an efficient enery dissipation process, called non-photochemical quenching (NPQ), in Chlamydomonas reinhardtii.

Product citations

Selected references Cazzaniga et al. (2022). Engineering astaxanthin accumulation reduces photoinhibition and increases biomass productivity under high light in Chlamydomonas reinhardtii. Biotechnol Biofuels Bioprod. 2022 Jul 11;15(1):77. doi: 10.1186/s13068-022-02173-3. PMID: 35820961; PMCID: PMC9277849.
Redekop et al. (2020). PsbS Contributes to Photoprotection in Chlamydomonas Reinhardtii Independently of Energy Dissipation . Biochim Biophys Acta Bioenerg . 2020 Jun 1;1861(5-6):148183.doi: 10.1016
Lammermann et al. (2020). Ubiquitin ligase component LRS1 and transcription factor CrHy5 act as a light switch for photoprotection in Chlamydomonas. doi.org/10.1101/2020.02.10.942334 bioRxiv
Roach et al. (2020). The non-photochemical quenching protein LHCSR3 prevents oxygen-dependent photoinhibition in Chlamydomonas reinhardtii. J Exp Bot. 2020 Jan 16. pii: eraa022. doi: 10.1093/jxb/eraa022.
Gabilly et al. (2019). Regulation of photoprotection gene expression in Chlamydomonas by a putative E3 ubiquitin ligase complex and a homolog of CONSTANS. Proc Natl Acad Sci U S A. 2019 Aug 12. pii: 201821689. doi: 10.1073/pnas.1821689116.
Tian et al. (2019). pH dependence, kinetics and light-harvesting regulation of nonphotochemical quenching in Chlamydomonas. Proc Natl Acad Sci U S A. 2019 Apr 23;116(17):8320-8325. doi: 10.1073/pnas.
Aihara et al. (2019). Algal photoprotection is regulated by the E3 ligase CUL4-DDB1DET1. Nat Plants. 2019 Jan;5(1):34-40. doi: 10.1038/s41477-018-0332-5.
Kosuge et al.(2018). LHCSR1-dependent fluorescence quenching is mediated by excitation energy transfer from LHCII to photosystem I in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):3722-3727. doi: 10.1073/pnas.1720574115.
Giovagnetti et al. (2018). A siphonous morphology affects light-harvesting modulation in the intertidal green macroalga Bryopsis corticulans (Ulvophyceae). Planta. 2018 Feb 19. doi: 10.1007/s00425-018-2854-5.
Chukhutsina et al. (2017). Photoprotection strategies of the alga Nannochloropsis gaditana. Biochim Biophys Acta. 2017 Jul;1858(7):544-552. doi: 10.1016/j.bbabio.2017.05.003.
Allorent et al. (2016). UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):14864-14869. doi: 10.1073/pnas.1607695114. Epub 2016 Dec 5.
Dinc et al. (2016). LHCSR1 induces a fast and reversible pH-dependent fluorescence quenching in LHCII in Chlamydomonas reinhardtii cells. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):7673-8. doi: 10.1073/pnas.1605380113. Epub 2016 Jun 22.
Correa-Galvis et al. (2016). Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii. J Biol Chem. 2016 Aug 12;291(33):17478-87. doi: 10.1074/jbc.M116.737312.

Confirmed reactivity:	Bryopsis corticulans, Chlamydomonas reinhardtii, Nannochloropsis gaditana
predicted reactivity:	Species of your interest not listed? Contact us
not reactive in:	Lobosphaera incisa, Phaeodactylum tricornutum
Picture (footer):	Application example 10 ug of a total cell extract of Chlamydomonas reinhardtii: WT _P _HL_High CO2 (1), WT_P_HL (2), Npq4_P _HL (3), were separated on Bolt® 4-12% Bis-Tris Plus Gels (precast) and blotted to PVDF using Bolt® transfer system for 1h . Blots were blocked with 5% BSA/milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10 seconds. P: photoautotrophically grown cells HL: High light illumination(light intensity:500 μE) Courtesy Dr. Roberta Croce, Biophysics of Photosynthesis Dep. Physics and Astronomy Faculty of Sciences VU University Amsterdam, The Netherlands
calculated \| apparent molecular mass [kDa]:	27 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide derived from LHCSR1 protein sequence from Chlamydomonas reinhardtii, UniProt: P93664
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Quantity:	50 µl
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
All references:	Cazzaniga et al. (2022). Engineering astaxanthin accumulation reduces photoinhibition and increases biomass productivity under high light in Chlamydomonas reinhardtii. Biotechnol Biofuels Bioprod. 2022 Jul 11;15(1):77. doi: 10.1186/s13068-022-02173-3. PMID: 35820961; PMCID: PMC9277849. Redekop et al. (2020). PsbS Contributes to Photoprotection in Chlamydomonas Reinhardtii Independently of Energy Dissipation . Biochim Biophys Acta Bioenerg . 2020 Jun 1;1861(5-6):148183.doi: 10.1016 Lammermann et al. (2020). Ubiquitin ligase component LRS1 and transcription factor CrHy5 act as a light switch for photoprotection in Chlamydomonas. doi.org/10.1101/2020.02.10.942334 bioRxiv Roach et al. (2020). The non-photochemical quenching protein LHCSR3 prevents oxygen-dependent photoinhibition in Chlamydomonas reinhardtii. J Exp Bot. 2020 Jan 16. pii: eraa022. doi: 10.1093/jxb/eraa022. Gabilly et al. (2019). Regulation of photoprotection gene expression in Chlamydomonas by a putative E3 ubiquitin ligase complex and a homolog of CONSTANS. Proc Natl Acad Sci U S A. 2019 Aug 12. pii: 201821689. doi: 10.1073/pnas.1821689116. Tian et al. (2019). pH dependence, kinetics and light-harvesting regulation of nonphotochemical quenching in Chlamydomonas. Proc Natl Acad Sci U S A. 2019 Apr 23;116(17):8320-8325. doi: 10.1073/pnas. Aihara et al. (2019). Algal photoprotection is regulated by the E3 ligase CUL4-DDB1DET1. Nat Plants. 2019 Jan;5(1):34-40. doi: 10.1038/s41477-018-0332-5. Kosuge et al.(2018). LHCSR1-dependent fluorescence quenching is mediated by excitation energy transfer from LHCII to photosystem I in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):3722-3727. doi: 10.1073/pnas.1720574115. Giovagnetti et al. (2018). A siphonous morphology affects light-harvesting modulation in the intertidal green macroalga Bryopsis corticulans (Ulvophyceae). Planta. 2018 Feb 19. doi: 10.1007/s00425-018-2854-5. Chukhutsina et al. (2017). Photoprotection strategies of the alga Nannochloropsis gaditana. Biochim Biophys Acta. 2017 Jul;1858(7):544-552. doi: 10.1016/j.bbabio.2017.05.003. Allorent et al. (2016). UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):14864-14869. doi: 10.1073/pnas.1607695114. Epub 2016 Dec 5. Dinc et al. (2016). LHCSR1 induces a fast and reversible pH-dependent fluorescence quenching in LHCII in Chlamydomonas reinhardtii cells. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):7673-8. doi: 10.1073/pnas.1605380113. Epub 2016 Jun 22. Correa-Galvis et al. (2016). Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii. J Biol Chem. 2016 Aug 12;291(33):17478-87. doi: 10.1074/jbc.M116.737312.
background:	LHCSR1 (Stress-related chlorophyll a/b binding protein 1) plays a role in an efficient enery dissipation process, called non-photochemical quenching (NPQ), in Chlamydomonas reinhardtii.
additional information (application):	This antibody is also recognizing recombinant LHCSR1 overexpressed in E.coli as described in Perozeni et al. (2020).