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Linamarase

AS15 2862 | Clonality: Polyclonal  | Host: Rabbit | Reactivity: Manihot esculentum

Linamarase in the group Antibodies Plant/Algal  / Carbohydrates at Agrisera AB (Antibodies for research) (AS15 2862)



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Product Information

Immunogen KLH-conjugated synthetic peptide derived from Manihot esculenta linamarase protein sequence, UniProt: O24524
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution

For reconstitution add 50 µl, of sterile water.

Storage

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution 1: 1000 (WB)
Expected | apparent MW 59 kDa

Reactivity

Confirmed reactivity Manihot esculenta
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-linamarase antibodies



Samples:
Latex - approximately 20 µl dissolved in 200 µl of extraction buffer
Leaf (cassava) - 2 leaf discs (A=78.5 mm2), snap frozen in liquid nitrogen and homogenized with pistil in 200 µl extraction buffer

10 µl of extract, is mixed with sample buffer (BioRadXT), reducing agent (BioRadXT) and miliQ water to a final volume of 35 µl - 30 µll of this extract is separated on 12 % Bis-Tris (BioRad Criterion) gel and run at 200 V for 1h in XT MOPS buffer. Blotting is done to PVDF membrane with BioRad Criterion, 100 V/30 min. Membranes are blocked in 5 % non-fat milk for 1h/RT followed by primary antibody incubation at 1: 1000 in 5 % non-fat milk in PBS-T for 1h/RT. Membrane is washed in PBS-T, 3x10 min. and incubated in anti-rabbit HRP conjugated secondary antibodies in PBS-T with 5 % non-fat milk for 2h/RT. Following the wash in PBS-T for 3x 10 min. signal is developed with chemiluminescent detection reagent and recorded by a CCD camera. 

 Multiple tissue analysis of linamarase

Linamarase was analyzed in multiple tissues by Western blot, using protocol described above.

Additional information

Related products

Background

Product citations

Selected references Linamarase is an enzyme found in cell walls of many plants, which converts the cyanide containing compounds into acetone cyanohydrin.
immunogen: KLH-conjugated synthetic peptide derived from Manihot esculenta linamarase protein sequence, UniProt: O24524
Reconstitution:

For reconstitution add 50 µl, of sterile water.

Host: Rabbit
Clonality: Polyclonal
Purity: Serum
Format: Lyophilized
Quantity: 50 µl
storage:

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications: Western blot (WB)
recommended dilution: 1: 1000 (WB)
calculated | apparent molecular mass [kDa]: 59 kDa
Confirmed reactivity: Manihot esculenta
predicted reactivity: Species of your interest not listed? Contact us
not reactive in:

no confirmed exceptions from predicted reactivity are currently known

Picture (footer): Western blot using anti-linamarase antibodies



Samples:
Latex - approximately 20 µl dissolved in 200 µl of extraction buffer
Leaf (cassava) - 2 leaf discs (A=78.5 mm2), snap frozen in liquid nitrogen and homogenized with pistil in 200 µl extraction buffer

10 µl of extract, is mixed with sample buffer (BioRadXT), reducing agent (BioRadXT) and miliQ water to a final volume of 35 µl - 30 µll of this extract is separated on 12 % Bis-Tris (BioRad Criterion) gel and run at 200 V for 1h in XT MOPS buffer. Blotting is done to PVDF membrane with BioRad Criterion, 100 V/30 min. Membranes are blocked in 5 % non-fat milk for 1h/RT followed by primary antibody incubation at 1: 1000 in 5 % non-fat milk in PBS-T for 1h/RT. Membrane is washed in PBS-T, 3x10 min. and incubated in anti-rabbit HRP conjugated secondary antibodies in PBS-T with 5 % non-fat milk for 2h/RT. Following the wash in PBS-T for 3x 10 min. signal is developed with chemiluminescent detection reagent and recorded by a CCD camera. 

 Multiple tissue analysis of linamarase

Linamarase was analyzed in multiple tissues by Western blot, using protocol described above.

All references: Linamarase is an enzyme found in cell walls of many plants, which converts the cyanide containing compounds into acetone cyanohydrin.

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