LUX | Transcription factor LUX

No confirmed exceptions from predicted reactivity are currently known.

Samples: 
1- 50 µg pEG202-LUX/Col-0 (FLAG-tag)
2- 50 µg Col-0
3- 50 µg lux mutant (SALK_119768) Expected product size: LUX (35kDa) and FLAG-LUX (35+1=36kDa)
All samples were 7-day-old seedlings grown under 22°C 16 light/8 dark.

50 µg/well of total protein extracted freshly from Arabidopsis thaliana seedlings. Exact buffer components were: and denatured with 100 mM Tris-HCl, pH7.8, 4 M Urea, 5% SDS, 15% glycerol, protease inhibitor cocktail, β-Mercaptoethanol, and bromophenol blue dye at 100°C/10 min. Samples were separated in the cold on 10% SDS-PAGE and blotted to Immobilon®-P PVDF Membrane PVDF (pore size of 0.45 µm), using: wet transfer in the cold. Blot was blocked with 5 % milk 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1:2000 for 1h with agitation in TBS-T with agitation. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit HRP conjugated) diluted to 1:25 000 in TBS-T for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent: Agrisera Super Bright (AS16 ECL-S-10). Exposure time was 5 sec.

Courtesy of Dr. Chin-Mei Lee, Institute of Plant Biology Global Agriculture Technology and Genomic Science Master Program, National Taiwan University, Taiwan

LUX (Transcription factor) essential for the generation of the circadian clock oscillation and activation of CCA1 and LHY expression. It is coregulated with TOC1 and seems to be repressed by CCA1 and LHY by direct binding of these proteins to the evening element in the LUX promoter. Binds to ELF3 and associates with ELF4 in a diurnal complex which is required for the expression of the growth-promoting transcription factors PIF4 and PIF5 and subsequent hypocotyl growth in the early evening.
Alternative names: Protein LUX ARRHYTHMO, Protein PHYTOCLOCK 1.