AS12 1842 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Solanum tuberosum
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75 µg of total protein from Solanum tuberosum v. Nicola extracted with 20 mM TRIS PH-8.5, 10 mM thiourea,10mM CaCl2, 5mM DTT, 1 mM PMSF, 1%PVPP were separated on 4-20 % SDS-PAGE and blotted 1.5h to PVDF. Blots were blocked with 5% milk powder in T-TBS (2.5gr of powder in 50 ml T-TBS) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for overnight at 4°C with agitation. The antibody solution was decanted and the blot was washed 6 times for 5 min. in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:10 000 in blocking solution for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 30 seconds.
Courtesy of Dr. Paula Teper-Bamnolker and Dr. Dani Eshel, The Volcani Center, Israel
Courtesy of Msc. Iauhenia Isayenka, University of Sherbrooke, Canada
Patatin is the most abundant protein in potato (Solanum tuberosum L.) tubers, about 60 % of a total protein. It serves as a storage protein. Mature tuber patatin variants are dimers of 40-42 kDa subunits without disulphide bridges.
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