Pectic polysaccharide, homogalacturonan (monoclonal, clone LM7)

Name: Pectic polysaccharide, homogalacturonan (monoclonal, clone LM7)
Brand: IKP
SKU: AS18 4196-1ml
Price: 246 EUR
Availability: InStock

AS18 4196-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom

Pectic polysaccharide, homogalacturonan (monoclonal, clone LM7) in the group Antibodies Plant/Algal / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4196-1ml)

Pectic polysaccharide, homogalacturonan (monoclonal, clone LM7)

Product Information

Immunogen Pectic polysaccharide, Homogalacturonan,

Host Rat

Clonality Monoclonal

Subclass/isotype IgM

Purity Cell culture supernatant.

Format Liquid

Quantity 1 ml

Storage Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunoflourescence (IF)

Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Contains 0.05% Sodium Azide

The antibody recognizes a partially methyl-estrified epitope of HG which results from non-blockwise de-estrification processes.
The antibody does not bind to un-estrified homogalacuronan.

Background

The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.

Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls

Product citations

Selected references Zhang et al. (2022) Mutation of CESA1 phosphorylation site influences pectin synthesis and methylesterification with a role in seed development, Journal of Plant Physiology, 2022, 153631, ISSN 0176-1617, https://doi.org/10.1016/j.jplph.2022.153631.

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immunogen:	Pectic polysaccharide, Homogalacturonan,
Sub class:	IgM
Host:	Rat
Clonality:	Monoclonal
Purity:	Cell culture supernatant.
Format:	Liquid
Quantity:	1 ml
storage:	Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.
Tested applications:	ELISA (ELISA), Immunoflourescence (IF)
recommended dilution:	1:10 (ELISA, IF)
Confirmed reactivity:	Higher plants, ferns and mosses
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Contains 0.05% Sodium Azide The antibody recognizes a partially methyl-estrified epitope of HG which results from non-blockwise de-estrification processes. The antibody does not bind to un-estrified homogalacuronan.
background:	The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls
All references:	Zhang et al. (2022) Mutation of CESA1 phosphorylation site influences pectin synthesis and methylesterification with a role in seed development, Journal of Plant Physiology, 2022, 153631, ISSN 0176-1617, https://doi.org/10.1016/j.jplph.2022.153631.