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Pectic polysaccharide, homogalacturonan (monoclonal, clone LM7)

AS18 4196-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses


Pectic polysaccharide, homogalacturonan (monoclonal, clone LM7) in the group Antibodies for Plant/Algal  / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4196-1ml)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen Pectic polysaccharide, Homogalacturonan.
Host Rat
Clonality Monoclonal
Subclass/isotype IgM
Format Cell Culture Supernatant
Quantity 1 ml
Storage Store at +4°C (short term) and at -20°C (long term).

Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunoflourescence (IF)
Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses.
Not reactive in No confirmed exceptions from predicted reactivity known at the moment.

Application examples

Additional information

Additional information Contains 0.05% Sodium Azide

The antibody recognizes a partially methyl-estrified epitope of HG which results from non-blockwise de-estrification processes.
The antibody does not bind to un-estrified homogalacuronan.

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Background

Background

The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.  

Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls

Product citations

Selected references Clausen et al. (2003). Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr Res. 003 Aug 12;338(17):1797-800.doi: 10.1016/s0008-6215(03)00272-6.
Willats et al. (2001). Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls. Implications for pectin methyl sterase action, matrix properties, and cell adhesion. j. Biiol Chem. 2001 Jun 1;276(22):19404-13.doi: 10.1074/jbc.M011242200.