Pectic polysaccharide, homogalacturonan (monoclonal, clone LM7)

AS18 4196-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom

Pectic polysaccharide, homogalacturonan (monoclonal, clone LM7)

Product Information

Immunogen Pectic polysaccharide, Homogalacturonan,

Host Rat

Clonality Monoclonal

Subclass/isotype IgM

Purity Cell culture supernatant.

Format Liquid

Quantity 1 ml

Storage Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunoflourescence (IF)

Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Contains 0.05% Sodium Azide

The antibody recognizes a partially methyl-estrified epitope of HG which results from non-blockwise de-estrification processes.
The antibody does not bind to un-estrified homogalacuronan.

Background

The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.

Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls

Product citations

Selected references Zhang et al. (2022) Mutation of CESA1 phosphorylation site influences pectin synthesis and methylesterification with a role in seed development, Journal of Plant Physiology, 2022, 153631, ISSN 0176-1617, https://doi.org/10.1016/j.jplph.2022.153631.

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Confirmed reactivity:	Higher plants, ferns and mosses
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Contains 0.05% Sodium Azide The antibody recognizes a partially methyl-estrified epitope of HG which results from non-blockwise de-estrification processes. The antibody does not bind to un-estrified homogalacuronan.
All references:	Zhang et al. (2022) Mutation of CESA1 phosphorylation site influences pectin synthesis and methylesterification with a role in seed development, Journal of Plant Physiology, 2022, 153631, ISSN 0176-1617, https://doi.org/10.1016/j.jplph.2022.153631.
background:	The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls
Clonality:	Monoclonal
Format:	Liquid
Host:	Rat
immunogen:	Pectic polysaccharide, Homogalacturonan,
Purity:	Cell culture supernatant.
Quantity:	1 ml
recommended dilution:	1:10 (ELISA, IF)
storage:	Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.
Sub class:	IgM
tested applications:	ELISA (ELISA), Immunoflourescence (IF)