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PIP2;1, PIP2;2, PIP2;3 | Plasma membrane intrinistic protein 2-1,2-2,2-3

AS09 491 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, G. coquereliana, R. sativus 

PIP2;1, PIP2;2, PIP2;3 | Plasma membrane intrinistic protein 2-1,2-2,2-3 in the group Antibodies for Plant/Algal  / Membrane Transport System / Plasma membrane at Agrisera AB (Antibodies for research) (AS09 491)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIP2 proteins: AtPIP2-1, At3g53420 , AtPIP2-2 P43287, At2g37170 , AtPIP2-3 P30302, At2g37180

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 8000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW

30.4 | 28 (PIP2-1,PIP2-2,PIP2-3) kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Camelina sativa, Gromphadorhina coquereliana, Raphanus sativus
Predicted reactivity Brassica napus, Cucumis sativus, Glycine hispida, Gossypium hirsutum, Hedychium coronarium, Mimosa saman, Nicotiana glauca, Petunia hybrida, Pisum sativum, Ricinus communis, Populus tremula x Populus tremloides, Physcomitrella patens
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot using anti-PIP2s antibodies

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP2s antibodies (AS09 491, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Additional information

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.

Related products

Related products

collection of antibodies to plasma membrane proteins

AS09 488 | anti-PIP2;1 | aquaporin PIP2;1

AS09 489 | anti-PIP1;1, PIP1;2, PIP1;3 | aquaporins

AS09 490 | anti-PIP2;2 | plasma membrane aquaporin 2b

Plant protein extraction buffer

Secondary antibodies

Background

Background

PIP2;2 is a plasma membrane aquaporin. Alternative names of isoforms: aquaporin PIP2-1, plasma membrane intrinsic protein 2a, PIP2a, aquaporin PIP2-2, plasma membrane intrinsic protein 2b, PIP2b, TMP2b, Aquaporin PIP2-3, plasma membrane intrinsic protein 2c, PIP2c, TMP2C, RD28-PIP, water stress-induced tonoplast intrinsic protein, (WSII-TIP)

Product citations

Selected references Hyun-Sung et al. (2019). NaCl-induced CsRCI2E and CsRCI2F interact with aquaporin CsPIP2;1 to reduce water transport in Camelina sativa L. Biochemical and Biophysical Research Communications,Available online 4 April 2019.
Chowanski et al. (2015). Cold induced changes in lipid, protein and carbohydrate levels in the tropical insect Gromphadorhina coquereliana. Comp Biochem Physiol A Mol Integr Physiol. 2015 May;183:57-63. doi: 10.1016/j.cbpa.2015.01.007. Epub 2015 Jan 23.

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