PntA (Slr1239) | Pyridine nucleotide transhydrogenase alpha-subunit

Product no: AS15 2910
AS15 2910 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. PCC 6803 
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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Synechocystis sp. PCC6803 NAD(P) transhydrogenase alpha subunit sequence, UniProt: A0A068N3D0

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 3000 (WB)
    Expected | apparent MW: 56 kDa
  • Reactivity
  • Confirmed reactivity: Synechocystis sp. PCC6803
    Predicted reactivity: Bacillus subtilis, Cyanothece sp. PCC 7822, Desulfobulbaceae bacterium BRH_c16a, Elusimicrobia bacterium, Fischerella sp. JSC-11, Hapalosiphon sp. MRB220, Magnetococcus marinus, Moorea producens JHB , Pleurocapsa sp. PCC 7327, Stanieria cyanosphaera
    Species of your interest not listed? Contact us
    Not reactive in: Algae
  • Application Examples
  • Application example

    Western blot using anti-PntA antibodies

    25 µg of total proteins from Synechocystis sp. PCC 6803 (WT) and the ΔpntA mutant extracted with buffer containing 50 mM HEPES-NaOH, pH 7.5, 30 mM CaCl2, 800 mM sorbitol and 1mM 6-aminohexanoid acid and denatured with 2xLaemli buffer at +4 °C for O/N. Proteins were separated on 12 % SDS-PAGE containing 6 M urea and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk for 1h at room temperature (RT) with agitation. After that blot was washed with TTBS for 2 x 5 min and incubated in the primary antibody at a dilution of 1: 3000 in TTBS for O/N at +4 °C with agitation. The antibody solution was decanted and the blot was rinsed briefly with TTBS and washed 4 x 5 min with TTBS at RT with agitation. Blot was incubated in secondary antibody (Amersham ECL Rabbit IgG, HRP-linked whole Ab, from GE Healthcare) diluted to 1:10 000 in for 2h at RT with agitation. The blot was washed 3 x 5 min with TTBS and 2 x 5 min with TBS and developed for 5 min with chemiluminescent detection reagent. Exposure time was around 2 min.

    Courtesy of Tuomas Huokko, University of Turku, Finland

  • Background
  • Background: PntA (Slr1239) (Pyridine nucleotide transhydrogenase alpha-subunit) is an integral mambrane protein complex which participates in the regulation of ion of NAD(P)+:NAD(P)H redox homeostasis. Functional enzyme is a dimer of PntA and PntB.
  • Product Citations
  • Selected references: Kämäräinen et al. (2017). Pyridine nucleotide transhydrogenase PntAB is essential for optimal growth and photosynthetic integrity under low-light mixotrophic conditions in Synechocystis sp. PCC 6803. New Phytol. 2017 Apr;214(1):194-204. doi: 10.1111/nph.14353.
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