PsbO1 | 33 kDa of the oxygen evolving complex (OEC) of PSII

AS14 2824 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana

PsbO1 | 33 kDa of the oxygen evolving complex (OEC) of PSII

Product Information

Immunogen

KLH-conjugated peptide chosen chosen from Arabidopsis thaliana PsbO1, UniProt: P23321, TAIR:At5g66570

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

33 | 30 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Brassica napus, Capsella rubella, Triticum aestivum
Species of your interest not listed? Contact us

Not reactive in Oryza sativa

Application examples

Application examples application example

western blot using anti-PsbO1 antibodies

30 µg of thylakoid protein from Arabidopsis thaliana Columbia-0 extracted with 330 mM sorbitol, 50 mM Hepes-KOH (pH 7.8) were separated on 15% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 6h at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 1% milk (TBS-T) for 2h at 4°C with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 60 seconds.

Courtesy of Dr. Peter Gollan, University of Turku, Finland

Additional information

This antibody is specific to PsbO1 and does not react with PsbO2 as confirmed on a deletion mutant

Background

The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.

Product citations

Selected references Shanmugabalaji et al. (2018). Chloroplast Biogenesis Controlled by DELLA-TOC159 Interaction in Early Plant Development. Curr Biol. 2018 Aug 20;28(16):2616-2623.e5. doi: 10.1016/j.cub.2018.06.006.

additional information (application):	This antibody is specific to PsbO1 and does not react with PsbO2 as confirmed on a deletion mutant
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Brassica napus, Capsella rubella, Triticum aestivum Species of your interest not listed? Contact us
not reactive in:	Oryza sativa
calculated \| apparent molecular mass [kDa]:	33 \| 30 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated peptide chosen chosen from Arabidopsis thaliana PsbO1, UniProt: P23321, TAIR:At5g66570
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunoprecipitation (IP), Western blot (WB)
All references:	Shanmugabalaji et al. (2018). Chloroplast Biogenesis Controlled by DELLA-TOC159 Interaction in Early Plant Development. Curr Biol. 2018 Aug 20;28(16):2616-2623.e5. doi: 10.1016/j.cub.2018.06.006.
Picture (footer):	application example 30 µg of thylakoid protein from Arabidopsis thaliana Columbia-0 extracted with 330 mM sorbitol, 50 mM Hepes-KOH (pH 7.8) were separated on 15% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 6h at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 1% milk (TBS-T) for 2h at 4°C with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 60 seconds. Courtesy of Dr. Peter Gollan, University of Turku, Finland
background:	The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.

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