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PsbO2 | 33 kDa of the oxygen evolving complex (OEC) of PSII

AS14 2825  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana

PsbO2 | 33 kDa of the oxygen evolving complex (OEC) of PSII in the group Antibodies for Plant/Algal  / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS14 2825)

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Product Information

Immunogen

KLH-conjugated peptide chosen chosen from Arabidopsis thaliana PsbO2, UniProt:Q9S841, TAIR: At3g50820

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

33 | 30 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica napus, Capsella rubella, dinoflagellate, Triticum aestivum
Species of your interest not listed? Contact us

Application examples

Application examples application example

western blot using anti-PsbO2 antibodies

30 µg of thylakoid protein from Arabidopsis thaliana Columbia-0 extracted with 330 mM sorbitol, 50 mM Hepes-KOH (pH 7.8) were separated on 15% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 6h at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 minutes and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 1% milk (TBS-T) for 2h at 4°C with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 60 seconds.

Courtesy of Dr. Peter Gollan, University of Turku, Finland

Additional information

This antibody is specific to PsbO2 and does not react with PsbO1 as confirmed on deletion mutant.

Related products

Related products

AS14 2824 | anti-PsbO1 | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS05 092 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibody

AS06 142-33 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibody

AS06 167 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibody

AS08 305 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibody

AS06 142-16 | anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Background

Background

The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include  33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II,  OEC 33 kDa subunit, 33 kDa thylakoid membrane protein,   manganese-stabilizing protein 2.

Product citations

Selected references Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4.

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