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PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-peptide)

AS06 167 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. tabacum, P.abies, P. sativum,  P. patens

PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-peptide) in the group Antibodies Plant/Algal  / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS06 167)
PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-peptide)



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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from PsbP protein of Arabidopsis thaliana Q42029, At1g06680

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

28 | 23 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Picea abies, Pisum sativum, Physcomitrium patens
Predicted reactivity Oryza sativa, Solanum tuberosum, Triticum aestivum, Picea sitchenisis, Populus balsamifera
Species of your interest not listed? Contact us
Not reactive in

Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942, Zostera marina

Application examples

Application examples

Application example

western blot using anti-PsbP

2 µg of total protein from (1) Arabidopsis thaliana leaf extracted with Protein Extration Buffer, PEB (AS08 300), (2) Hordeum vulgare leaf extracted with PEB, (3) Chlamydomonas reinhardtii total cell extracted with PEB, (4) Synechococcus sp. 7942 total cell extracted with PEB, were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min withchemiluminescent detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

Reactant: Arabidopsis thaliana (Thale cress)

Application: Western Blotting

Pudmed ID: 28791032

Journal: Front Plant Sci

Figure Number: 2A

Published Date: 2017-08-10

First Author: Kohzuma, K., Froehlich, J. E., et al.

Impact Factor: 5.435

Open Publication

Changes in the protein levels of photosynthetic components under extended dark exposure in wild-type and gamera-1. Immunoblot detection of photosynthetic proteins from leaves of Ws and gamera-1 plants incubated after dark adaptation for 0, 2, and 4 days was examined. Specifically, essentially thylakoid fractions were assayed to determine the content of the following proteins: ?-subunit of ATP synthase; the D1 protein, OEC17, OEC23, and OEC33 of PSII; Cyt f and Rieske protein of the cytochrome b6f complex; and the F and D subunits of PSI, after extended dark treatment. Proteins were resolved via SDS-PAGE gel based on equal microgram chlorophyll per lane loading and processed as described in Section “Materials and Methods”. The Large subunit of RuBisco and LHCII stained with either CBB or Ponceau red, respectively, are presented here as loading controls. DAD indicates days after dark adaptation.

Additional information

Additional information This product can be sold containing ProClin if requested
If you work with Chlamydomonas reinhardii, please use following PsbP antibody: AS06 142-23

Related products

Related products

AS05 092 | Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS06 142-33 | Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS08 305 | Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS06 142-16 | Anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

PsbP - 23 kDa extrinsic protein of photosystem II (PSII). Processing of the protein results in a protein with molecular mass of around 20 kDa. PsbP is required to optimize water splitting process in PSII, by probaböy by optimisation of calcium and Cl- levels. The protein is found in higher plants and algae but is not conserved in cyanobacteria. Synonymes:Oxygen-evolving enhancer protein 2-1, chloroplastic, OEE2, 23 kDa subunit of oxygen evolving system of photosystem II, OEC 23 kDa subunit, OEC23, 23 kDa thylakoid membrane protein

Product citations

Selected references Mazur et al. (2021) The SnRK2.10 kinase mitigates the adverse effects of salinity by protecting photosynthetic machinery. Plant Physiol. 2021 Dec 4;187(4):2785-2802. doi: 10.1093/plphys/kiab438. PMID: 34632500; PMCID: PMC8644180.
Tamburino et al. (2017). Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.). BMC Plant Biol. 2017 Feb 10;17(1):40. doi: 10.1186/s12870-017-0971-0.
Pavlovi? et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.
Albanese et al. (2016). Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy. Photosynth Res. 2016 Jan 9.
Grassl et al. (2012). Early events in plastid protein degradation in stay-green Arabidopsis reveal differential regulation beyond the retention of LHCII and chlorophyll. J. Proteome Res. October 2.
Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).

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