PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-monocot protein)

Product no: AS08 305

AS08 305  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, S. bicolor, Z. mays

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  • Product Info
  • Immunogen:

    Recombinant sorghum OE23 protein

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 l
    Reconstitution: For reconstitution add 100 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 5 000 (WB)
    Expected | apparent MW:

    23 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Sorghum bicolor, Zea mays
    Predicted reactivity: Hordeum vulgare, Oryza sativa
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    AS08 305 PsbP western.jpg

     
     
    5 µg of total protein from (1) Arabidopsis thaliana total leaf extract; (2) Zea mays total leaf  extract were separated in a 12% or 5-15% gradient gel following by transfer to nitrocellulose membrane. Membrane was blocked with TBST + 4% non-fat dried milk, 20 min following by three washes in TBST. Incubation time with primary and secondary antibodies was 1 hr primary, 30 min for secondary antibodies. Dectetion was performed using chemiluminescence. 
  • Background
  • Background:

    PSII reaction centre components are  generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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AS06 142-33  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A.thaliana, C. reinhardtii, C. vulgaris, C. sp. DT, E. crus-galli, F. arundinacea cv. Kord, H. pluvialis, H. spontaneum, H. vulgare, N. benthamiana, P. abies, P. banksiana, P. sativum, P. miliaceum, S. oleracea, S. tuberosum cultivar Taedong Valley, Synechococcus sp. PCC 7942 and PCC6803, T. salsuginea,
T.aestivum

compartment marker of thylakoid lumen Replaced by AS05 092

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