RBX1A | RING-box protein 1a

AS22 4828   | Clonality: Polyclonal|  Host: Rabbit | Reactivity: Arabidopsis Thaliana, Brassica napus, Nicotiana tabacum, Solanum lycopresicum, Solanum tuberosum, Hordeum vulgare, Oryza sativa, Zea mays, Trifolium pratense, Solanum verrucosum, Dendrobium catenatum, Zingiber officinale, Musa troglodytarum, Panicum miliaceum, Dioscorea cayenensis subsp. Rotundata, Impatiens glandulifera, Cicer arietinum

RBX1A | RING-box protein 1a in the group Antibodies Plant/Algal  / Brassica napus at Agrisera AB (Antibodies for research) (AS22 4828)

Data sheet Protocols Add review
calculated | apparent molecular mass [kDa]: | kDa (due to N-terminal or C-terminal processing)






immunogen: KLH-conjugated peptide derived from Q940X7
Purity: Affinity purified serum, in PBS pH 7.4
Quantity: 50 µg
recommended dilution: 1 : 1000 (WB)
Reconstitution: For reconstitution add 50 µl, of sterile or deionized water.
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications: Western blot (WB)
Picture (footer): application example

µg of total protein extracted freshly from    with write exact buffer components ..... and denatured °C or 70°C for 2-5 min.... were separated on  % SDS-PAGE  and blotted 1h to PVDF/nitrocellulose using wet, semi-dry or dry transfer. Blot was blocked with % milk or % BSA  for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in  for 1h/RT with agitation. The blot was washed as above and developed for   min with   name and supplier. Exposure time was.....seconds.

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Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Brassica napus
Nicotiana tabacum
Solanum lycopresicum
Solanum tuberosum
Hordeum vulgare
Oryza sativa
Zea mays
Trifolium pratense
Solanum verrucosum
Dendrobium catenatum
Zingiber officinale
Musa troglodytarum
Panicum miliaceum
Dioscorea cayenensis subsp. Rotundata
Impatiens glandulifer
aCicer arietinum

Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
background: Background info.

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