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Anti-RBX1A | RING-box protein 1a
Product no: AS22 4828
AS22 4828 | Clonality: Polyclonal| Host: Rabbit | Reactivity: Arabidopsis thaliana, Oryza sativa
329
€
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Delivery:
3-6 business days
- Product Info
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Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana RBX1A protein, UniProt: Q940X7 TAIR: At5g20570 Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 13 | 16 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Oryza sativa Predicted reactivity: Brassica napus, Cicer arietinum, Dendrobium catenatum, Dioscorea cayenensis subsp. Rotundata, Hordeum vulgare, Impatiens glandulifer, Musa troglodytarum, Nicotiana tabacum, Panicum miliaceum, Solanum lycopresicum, Solanum tuberosum, Solanum verrucosum, Trifolium pratense, Zea mays, Zingiber officinale
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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The total protein content of 7-day-old seedlings of Oryza sativa were extracted using an extraction buffer containing 50 mM Tris pH 7.5, 150 Mm NaCl, 0.5% Triton X-100, and a complete protease inhibitor (Sigma) (0.2 g of sample plus 100 ml of extraction solution). Cellular debris was removed by centrifugation at 4℃, 12,000rpm for 5 minutes, and the supernatant was mixed with the 4×Laemmli buffer (250 mM Tris pH 6.8, 8% SDS, 40% glycerol, 4% β-mercaptoethanol, 0.01% bromophenol blue) in a ratio of 3:1. After mixing, it is denatured at 95℃ for 5 minutes, followed by centrifugation at 12,000 rpm for 5 minutes. The supernatant is then for Western blotting. Proteins were separated using 10% SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF) membranes, and then, the PVDF membrane was blocked with 5% milk in TBST (0.8% NaCl, 0.02% KCl, 0.3% Tris, pH 7.4, and 0.05% Tween 20) for an hour at room temperature. Excess milk was washed off with TBST. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 3 h/RT with agitation in TBST. The antibody solution was decanted and the blot was rinsed briefly, then washed three times for 15 min in TBST at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG) diluted to 1:5 000 for 2 h/RT with agitation. The blot was washed three times for 15 min in TBST. Exposure time was 120 seconds at full resolution (Bio-Rad, ChemiDoc system). - Background
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Background: RBX1A (RING-box protein 1a) is a component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The SCF complex plays a crucial role in regulating response to auxin and is essential for growth and development. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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