Name:
Phone:
E-mail:
Address:






RGL2 | RGA-like protein 2 (DELLA protein)

AS11 1803 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

RGL2 | RGA-like protein 2 (DELLA protein) in the group Antibodies Plant/Algal  / Hormones / Biosynthesis/regulation at Agrisera AB (Antibodies for research) (AS11 1803)
RGL2 | RGA-like protein 2 (DELLA protein)



DATA SHEET IN PDF

Qty: 
302
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RGL2 protein, UniProt: Q8GXW1, TAIR: At3g03450

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 1000 (WB), transfected Arabidopsis thaliana protoplasts
Expected | apparent MW

60 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in

Brassica napus, Populus sp., Triticum sp.

Application examples

Additional information

As this protein is expressed in tissue in very low levels, please load at least 50 µg/well.
Recommended extraction and denaturation protocol: 

Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95°C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade.

Related products

Background

Background

DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal, stamen and anther development. Predominantly expressed in germinating seeds, flowers and siliques.  Synonymes: GRAS family protein 15, AtGRAS-15, Scarecrow-like protein 19, AtSCL19.

Product citations

Confirmed reactivity: Arabidopsis thaliana
not reactive in:

Brassica napus, Populus sp., Triticum sp.

additional information (application):

As this protein is expressed in tissue in very low levels, please load at least 50 µg/well.
Recommended extraction and denaturation protocol: 

Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95°C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade.
background:

DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal, stamen and anther development. Predominantly expressed in germinating seeds, flowers and siliques.  Synonymes: GRAS family protein 15, AtGRAS-15, Scarecrow-like protein 19, AtSCL19.

calculated | apparent molecular mass [kDa]:

60 kDa

Clonality: Polyclonal
Format: Lyophilized
Host: Rabbit
immunogen:

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RGL2 protein, UniProt: Q8GXW1, TAIR: At3g03450

Purity: Immunogen affinity purified serum in PBS pH 7.4.
Quantity: 50 ĩg
recommended dilution: 1 : 1000 (WB), transfected Arabidopsis thaliana protoplasts
Reconstitution: For reconstitution add 50 ĩl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Immunolocalization (IL), Western blot (WB)

Related products: RGL2 | RGA-like protein 2 (DELLA protein)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 26 €
AS13 2638 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
302 €
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...
201 €
AS16 3955 | Clonality: Polyclonal | Host: Goat | Reactivity: Arabidopsis thaliana
302 €
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items