RGL2 | RGA-like protein 2 (DELLA protein)

Product no: AS11 1803

AS11 1803 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RGL2 protein, UniProt: Q8GXW1, TAIR: At3g03450

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunolocalization (IL), Western blot (WB)
    Recommended dilution: 1 : 1000 (WB), transfected Arabidopsis thaliana protoplasts
    Expected | apparent MW:

    60 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Not reactive in:

    Brassica napus, Populus sp., Triticum sp.

  • Additional Information
  • Additional information (application):

    As this protein is expressed in tissue in very low levels, please load at least 50 µg/well.
    Recommended extraction and denaturation protocol: 

    Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95°C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade.
  • Background
  • Background:

    DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal, stamen and anther development. Predominantly expressed in germinating seeds, flowers and siliques.  Synonymes: GRAS family protein 15, AtGRAS-15, Scarecrow-like protein 19, AtSCL19.

  • Protocols


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