RGL2 | RGA-like protein 2 (DELLA protein)

Name: RGL2 | RGA-like protein 2 (DELLA protein)
SKU: AS11 1803
Price: 302 EUR
Availability: InStock

AS11 1803 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

RGL2 | RGA-like protein 2 (DELLA protein) in the group Antibodies Plant/Algal / Hormones / Biosynthesis/regulation at Agrisera AB (Antibodies for research) (AS11 1803)

RGL2 | RGA-like protein 2 (DELLA protein)

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RGL2 protein, UniProt: Q8GXW1, TAIR: At3g03450

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunolocalization (IL), Western blot (WB)

Recommended dilution 1 : 1000 (WB), transfected Arabidopsis thaliana protoplasts

Expected | apparent MW

60 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Not reactive in

Brassica napus, Populus sp., Triticum sp.

Application examples

Additional information

As this protein is expressed in tissue in very low levels, please load at least 50 µg/well.
Recommended extraction and denaturation protocol:

Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95°C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade.

Background

DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal, stamen and anther development. Predominantly expressed in germinating seeds, flowers and siliques. Synonymes: GRAS family protein 15, AtGRAS-15, Scarecrow-like protein 19, AtSCL19.

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Confirmed reactivity:	Arabidopsis thaliana
not reactive in:	Brassica napus, Populus sp., Triticum sp.
additional information (application):	As this protein is expressed in tissue in very low levels, please load at least 50 µg/well. Recommended extraction and denaturation protocol: Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95°C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade.
background:	DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal, stamen and anther development. Predominantly expressed in germinating seeds, flowers and siliques. Synonymes: GRAS family protein 15, AtGRAS-15, Scarecrow-like protein 19, AtSCL19.
calculated \| apparent molecular mass [kDa]:	60 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RGL2 protein, UniProt: Q8GXW1, TAIR: At3g03450
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Quantity:	50 µg
recommended dilution:	1 : 1000 (WB), transfected Arabidopsis thaliana protoplasts
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunolocalization (IL), Western blot (WB)