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RGL2 | RGA-like protein 2 (DELLA protein)

AS11 1803 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

RGL2 | RGA-like protein 2 (DELLA protein) in the group Plant/Algal Antibodies / Hormones / Biosynthesis/regulation at Agrisera AB (Antibodies for research) (AS11 1803)

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product information
Background

DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal, stamen and anther development. Predominantly expressed in germinating seeds, flowers and siliques.  Synonymes: GRAS family protein 15, AtGRAS-15, Scarecrow-like protein 19, AtSCL19.

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RGL2 protein, UniProt: Q8GXW1, TAIR: At3g03450

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
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Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB), transfected Arabidopsis thaliana protoplasts
Expected | apparent MW

60 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity
Not reactive in

Triticum sp.

Additional information

As this protein is expressed in tissue in very low levels, please load at least 50 µg/well.
Recommended extraction and denaturation protocol: 

Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95°C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade.
Selected references


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