S6K1-2 | Ribosomal-protein S6 kinase homolog 1,2 - phosphorylated

AS13 2664  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

S6K1-2 | Ribosomal-protein S6 kinase homolog 1,2 - phosphorylated in the group Antibodies Plant/Algal  / Environmental Stress / Cold stress at Agrisera AB (Antibodies for research) (AS13 2664)
S6K1-2 | Ribosomal-protein S6 kinase homolog 1,2 - phosphorylated


Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information


KLH-conjugated peptide containing phospho-Thr, derived from Arabidopsis thaliana S6K1: UniProt: P42818, TAIR: AT3G08730 and S6K2: UniProt: Q39030, TAIR: AT3G08720. Due to high amino acid homology, chosen peptide is conserved in both proteins:  S6K1 and S6K2.

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Never Store this antibody in 4°C
Tested applications Western blot (WB)
Recommended dilution 1 : 500 (WB)
Expected | apparent MW 52,6 kDa (S6K1) and 53 kDa (S6K2) | 60 kDa


Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Thelungiella halophila
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Application example

western blot using anti-S6K1-2 | Ribosomal-protein S6 kinase homolog 1,2 - phosphorylated antibodies

30 µg of total protein of Arabidopsis thaliana 10 days old seedlings extracted with Lacus buffer with or without phosphatase inhibitors and kept at 4°C prior to denaturation for 5 min. at 95°C. Samples were separated on 6.5 % SDS-PAGE and blotted 2h to PVDF. Phosphatase treatment was done with CIAP ("+"  phosphatase inhibitors added, "-" phosphatase inhibitors not included). Blots were blocked with 5% milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:500 for overnight at 4C with agitation. The antibody solution was decanted and the blot was washed three times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated from Agrisera, AS09 602) diluted to 1:10 000 in 2.5% milk in TBST for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was few minutes.

Courtesy of Dr. Rossana Henriques, CRAG, Spain

Additional information

Related products



Ribosomal S6 kinase 1/2 (S6K1/2) involved in TOR signaling pathway, in osmotic stress response. Activated by PDK1 and repressed during osmotic stress. Expressed in all tissues, especially during high metabolic activity in growing buds, root tips, leaf margins and germinating seeds. Alternative names: AtPK1/AtPK6 S6K1), AtPK2/AtPK19 (S6K2).

Product citations

Selected references González-López et al. (2021). Growth promotion in Arabidopsis thaliana by bacterial cyclodipeptides involves the TOR/S6K pathway activation. Journal of Plant Physiology. Volume 257, 2021, 153343,ISSN 0176-1617,
Kazibwe et al. (2020). TOR mediates the autophagy response to altered nucleotide homeostasis in a ribonuclease mutant. J Exp Bot. 2020 Sep 9;eraa410.doi: 10.1093/jxb/eraa410.
Dealy et al. (2019). CEP3 levels affect starvation-related growth responses of the primary root. J Exp Bot. 2019 Jun 6. pii: erz270. doi: 10.1093/jxb/erz270.
Wang et al. (2017). The inhibition of protein translation mediated by AtGCN1 is essential for cold tolerance in Arabidopsis thaliana. Plant Cell Environ. 2017 Jan;40(1):56-68. doi: 10.1111/pce.12826.
Wang et al. (2017). Reciprocal Regulation of the TOR Kinase and ABA Receptor Balances Plant Growth and Stress Response. Mol Cell. 2017 Dec 27. pii: S1097-2765(17)30930-9. doi: 10.1016/j.molcel.2017.12.002.

Related products: S6K1-2 | Ribosomal-protein S6 kinase homolog 1,2 - phosphorylated

AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volum...
From 16 €
AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
206 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
201 €
AS12 1855 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
302 €
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items