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Toc159 | Chloroplast outer envelope membrane translocon complex protein

AS07 239  |  Clonality: Polyclonal  |  Host: Guinea pig  |  Reactivity:  Pisum sativum

Toc159 | Chloroplast outer envelope membrane translocon complex protein in the group Antibodies Plant/Algal / Photosynthesis / Chaperones/Transporters at Agrisera AB (Antibodies for research) (AS07 239)
Toc159 | Chloroplast outer envelope membrane translocon complex protein



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Product Information

Immunogen

Recombinant Toc159 (C-terminally His-tagged, including the central GTPase domain but lacking the N-terminal acidic domain as well as the C-terminal membrane associating domain) from Pisum sativum Toc159 protein sequence (Q9LKR1)
Host Guinea pig
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 500 (CM), 1 : 10 000 (WB)
Expected | apparent MW 150 kDa (with protease inhibitors), 86 and 53 kDa (following proteolysis) and approx, 29 kDa degradation product (cross reaction with GTPase domain)

Reactivity

Confirmed reactivity Pisum sativum
Predicted reactivity

Dicots, Nicotiana benthamiana, Oryza sativa


Species of your interest not listed? Contact us
Not reactive in

Arabidopsis thaliana

Application examples

Additional information

Additional information Antibodies have been used in fluorescence and confocal microscopy and total internal reflectance confocal microscopy

Toc159 is extremely sensitive to proteolytic processing in vivo therefore use of protease inhibitors is of crucial importance. 

Related products

Background

Background

Toc159 is located in the outer chloroplast membrane and part of of the Toc/TOC (translocon of the outer envelope membrane of chloroplasts) complex. It mediates the GTP-dependent initial stages of preprotein import into chloroplasts. In Arabidopsis thaliana, Toc159 is encoded by a small gene family: atTOC159, atTOC132, atTOC120, and atTOC90. Phylogenetic analysis suggested that at least two distinct Toc159 subtypes, characterized by atToc159 and atToc132/atToc120, exist in plants.

Product citations

Selected references Wu et al. (2018). Control of Retrograde Signaling by Rapid Turnover of GENOMES UNCOUPLED 1. Plant Physiol. 2018 Jan 24. pii: pp.00009.2018. doi: 10.1104/pp.18.00009.
calculated | apparent molecular mass [kDa]: 150 kDa (with protease inhibitors), 86 and 53 kDa (following proteolysis) and approx, 29 kDa degradation product (cross reaction with GTPase domain)
Clonality: Polyclonal
Format: Lyophilized
Host: Guinea pig
immunogen:

Recombinant Toc159 (C-terminally His-tagged, including the central GTPase domain but lacking the N-terminal acidic domain as well as the C-terminal membrane associating domain) from Pisum sativum Toc159 protein sequence (Q9LKR1)
Purity: Serum
Quantity: 50 µl
recommended dilution: 1 : 500 (CM), 1 : 10 000 (WB)
Reconstitution: For reconstitution add 50 µl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Immunolocalization (IL), Western blot (WB)
All references: Wu et al. (2018). Control of Retrograde Signaling by Rapid Turnover of GENOMES UNCOUPLED 1. Plant Physiol. 2018 Jan 24. pii: pp.00009.2018. doi: 10.1104/pp.18.00009.
background:

Toc159 is located in the outer chloroplast membrane and part of of the Toc/TOC (translocon of the outer envelope membrane of chloroplasts) complex. It mediates the GTP-dependent initial stages of preprotein import into chloroplasts. In Arabidopsis thaliana, Toc159 is encoded by a small gene family: atTOC159, atTOC132, atTOC120, and atTOC90. Phylogenetic analysis suggested that at least two distinct Toc159 subtypes, characterized by atToc159 and atToc132/atToc120, exist in plants.
additional information: Antibodies have been used in fluorescence and confocal microscopy and total internal reflectance confocal microscopy
additional information (application):

Toc159 is extremely sensitive to proteolytic processing in vivo therefore use of protease inhibitors is of crucial importance. 
Confirmed reactivity: Pisum sativum
predicted reactivity:

Dicots, Nicotiana benthamiana, Oryza sativa


Species of your interest not listed? Contact us
not reactive in:

Arabidopsis thaliana

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