Toc159 | Chloroplast outer envelope membrane translocon complex protein

Recombinant Toc159 (C-terminally His-tagged, including the central GTPase domain but lacking the N-terminal acidic domain as well as the C-terminal membrane associating domain) from Pisum sativum Toc159 protein sequence (Q9LKR1)

150 kDa (with protease inhibitors), 86 and 53 kDa (following proteolysis) and approx. 29 kDa degradation product (cross reaction with GTPase domain)

Dicots, Oryza sativa

Arabidopsis thaliana

Antibodies have been used in fluorescence and confocal microscopy and total internal reflectance confocal microscopy.

Toc159 is extremely sensitive to proteolytic processing in vivo therefore use of protease inhibitors is of crucial importance. 

AS06 150  |  Anti-Toc75 (monocots and dicots) antibodies

AS08 292  |  Anti-Toc75 (Pisum sativum) antibodies

Toc159 is located in the outer chloroplast membrane and part of of the Toc/TOC (translocon of the outer envelope membrane of chloroplasts) complex. It mediates the GTP-dependent initial stages of preprotein import into chloroplasts. In Arabidopsis thaliana, Toc159 is encoded by a small gene family: atTOC159, atTOC132, atTOC120, and atTOC90. Phylogenetic analysis suggested that at least two distinct Toc159 subtypes, characterized by atToc159 and atToc132/atToc120, exist in plants.