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Anti-Toc159 | Chloroplast outer envelope membrane translocon complex protein
AS07 239 | Clonality: Polyclonal | Host: Guinea pig | Reactivity: Pisum sativum
- Product Info
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Immunogen: Recombinant Toc159 (C-terminally His-tagged, including the central GTPase domain but lacking the N-terminal acidic domain as well as the C-terminal membrane associating domain) from Pisum sativum Toc159 protein sequence (Q9LKR1)
Host: Guinea pig Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunolocalization (IL), Western blot (WB) Recommended dilution: 1 : 500 (CM), 1 : 10 000 (WB) Expected | apparent MW: 150 kDa (with protease inhibitors), 86 and 53 kDa (following proteolysis) and approx, 29 kDa degradation product (cross reaction with GTPase domain) - Reactivity
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Confirmed reactivity: Pisum sativum Predicted reactivity: Dicots, Nicotiana benthamiana, Oryza sativa
Species of your interest not listed? Contact usNot reactive in: Arabidopsis thaliana
- Additional Information
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Additional information: Antibodies have been used in fluorescence and confocal microscopy and total internal reflectance confocal microscopy Additional information (application): Toc159 is extremely sensitive to proteolytic processing in vivo therefore use of protease inhibitors is of crucial importance.
- Background
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Background: Toc159 is located in the outer chloroplast membrane and part of of the Toc/TOC (translocon of the outer envelope membrane of chloroplasts) complex. It mediates the GTP-dependent initial stages of preprotein import into chloroplasts. In Arabidopsis thaliana, Toc159 is encoded by a small gene family: atTOC159, atTOC132, atTOC120, and atTOC90. Phylogenetic analysis suggested that at least two distinct Toc159 subtypes, characterized by atToc159 and atToc132/atToc120, exist in plants.
- Product Citations
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Selected references: Wu et al. (2018). Control of Retrograde Signaling by Rapid Turnover of GENOMES UNCOUPLED 1. Plant Physiol. 2018 Jan 24. pii: pp.00009.2018. doi: 10.1104/pp.18.00009. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Accessories
AS07 238 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Chalmydomonas reinhardtii
AS10 653 | Clonality: Polyclonal | Host: Goat | Reactivity: Guinea pig IgG (H&L)
AS07 235 | Clonality: Polyclonal | Host: Hen | Reactivity: Pisum sativum
AS07 236 | Clonality: Polyclonal | Host: Hen | Reactivity: Arabidopsis thaliana