V-ATPase, B | vacuolar H+-ATPase subunit B
AS14 2775 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Vigna radiata

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Product Information
KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana V-ATPase subunit B, isoform B1: UniProt: Q683E8, TAIR: AT1G76030, isoform B2 UniProt: Q9SZN1, TAIR: AT4G38510, isoform B3: UniProt: Q8W4E2 , TAIR: AT1G20260
53 | 57 kDa (Vigna radiata)
Reactivity
Acetabularia sp. , Arundo donax, Chlamydomonas reinhardtii, Cucumis sativus, Glycine soja, Gossypium mexicanum, Halostachys caspica, Haloxylon ammodendron, Hordeum vulgare, Medicago truncatula, Mesembryantheum crystallinum, Ostreococcus tauri, Oryza sativa, Panax ginseng, Physcomitrium patens, Pinus sylvestris, Populus trichocarpa, Pyrus sp., Ricinus communis, Theobroma cacao, Triticum aestivum, Zea mays, Zostera marina
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Application examples

Proteins were separated by SDS-PAGE and transferred to an Immobilon-P membrane (Millipore) using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) with transfer buffer (100 mM Tris, 192 mM Glycine, 0.02% (w/v) SDS and 5% (v/v) methanol). After treatment with 1% blocking agent, the membrane filter was incubated with the primary antibody (1:1000) and then with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (H+L) (Agrisera AS09 602, 1:25 000). Chemiluminescent reagent was used for detection of antigens. Chemiluminescence was detected with a Light-Capture II imaging device with a cooled CCD camera (Atto).
Samples:
1: 10 μg of 100,000 x g precipitate prepared from Arabidopsis thaliana 6 weeks old shoot.
2: 0.2 μg of vacuolar membrane enriched fraction prepared from Arabidopsis thaliana 6 weeks old shoot.
3: 2 μg of vacuolar membrane enriched fraction prepared from Arabidopsis thaliana 6 weeks old shoot.
4: 0.2 μg of vacuolar membrane enriched fraction prepared from Vigna radiata 4 days old hypocotyls. 5: 2 μg of vacuolar membrane enriched fraction prepared from Vigna radiata 4 days old hypocotyls.
Courtesy of Drs. Masayoshi Maeshima and Dr Shoji Segami, Nagoya University, Japan
Additional information
Background
V-ATPase, subunit B is a non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. Alternative names: Vacuolar proton pump subunit B1, vacuolar H+-ATPase subunit B, V-ATPase 57 kDa subunit.
Product citations
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