V-ATPase, B | vacuolar H+-ATPase subunit B

Product no: AS14 2775

AS14 2775 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Vigna radiata

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana V-ATPase subunit B, isoform B1: UniProt: Q683E8, TAIR: AT1G76030, isoform B2 UniProt: Q9SZN1, TAIR: AT4G38510, isoform B3: UniProt: Q8W4E2 , TAIR: AT1G20260

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    53 | 57 kDa (Vigna radiata)

  • Reactivity
  • Confirmed reactivity: Arabidopsis halleri, Nicotiana tabaccum, Thellungiella salsuginea, Vigna radiata
    Predicted reactivity:

    Acetabularia sp. , Arundo donax, Chlamydomonas reinhardtii, Cucumis sativus, Glycine soja, Gossypium mexicanum, Halostachys caspica,  Haloxylon ammodendron, Hordeum vulgare, Medicago truncatula, Mesembryantheum crystallinum, Ostreococcus tauri, Oryza sativa, Panax ginseng, Physcomitrium patens, Pinus sylvestris, Populus trichocarpa, Pyrus sp., Ricinus communis, Theobroma cacao, Triticum aestivum, Zea mays, Zostera marina


    Species of your interest not listed? Contact us
    Not reactive in: Thermatoga neapolitana
  • Application Examples
  • Applicaiton example


    western blot using anti-V-ATPase subunit B antibodies

    Proteins were separated by SDS-PAGE and transferred to an Immobilon-P membrane (Millipore) using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) with transfer buffer (100 mM Tris, 192 mM Glycine, 0.02% (w/v) SDS and 5% (v/v) methanol). After treatment with 1% blocking agent, the membrane filter was incubated with the primary antibody (1:1000) and then with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (H+L) (Agrisera AS09 602, 1:25 000). Chemiluminescent reagent was used for detection of antigens. Chemiluminescence was detected with a Light-Capture II imaging device with a cooled CCD camera (Atto).

    Samples:
    1: 10 μg of 100,000 x g precipitate prepared from Arabidopsis thaliana 6 weeks old shoot.
    2: 0.2 μg of vacuolar membrane enriched fraction prepared from Arabidopsis thaliana 6 weeks old shoot.
    3: 2 μg of vacuolar membrane enriched fraction prepared from Arabidopsis thaliana 6 weeks old shoot.
    4: 0.2 μg of vacuolar membrane enriched fraction prepared from Vigna radiata 4 days old hypocotyls. 5: 2 μg of vacuolar membrane enriched fraction prepared from Vigna radiata 4 days old hypocotyls.

    Courtesy of Drs. Masayoshi Maeshima and Dr Shoji Segami, Nagoya University, Japan
  • Additional Information
  • Additional information: Antibodies will detect target protein in a few µg of a crude preparation loaded per well, If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient
    Additional information (application): Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel
  • Background
  • Background:

    V-ATPase, subunit B is a non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. Alternative names: Vacuolar proton pump subunit B1, vacuolar H+-ATPase subunit B, V-ATPase 57 kDa subunit.

  • Protocols
  • Agrisera Western Blot protocol and video tutorials



    Method for isolation of plant vacuolar membranes

    method for vacuolar membrane isolation

    Courtesy of Dr. Masayoshi Maeshima, Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences Nagoya University Nagoya, Japan

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AS07 213  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Higher plants including A.comosus, A.thaliana, C.sativus, C. australis R.Br, C.reinhardtii, F. margarita Swingle, H.vulgare, L.esculentum, L.longiflorum, Malus x domestica Borkh. c.v. Fuji, M. truncatula, M.crystallinum, N.tabacum, N.caerulescens, O.sativa, P.hybrida cv. Mitchell, Populus sp., P.vittata, Thellungiella sp., T. aestivum, Z.mays, V. vinifera |  Cellular [compartment marker] of tonoplast membrane

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