What are the benefits of using directly conjugated primary antibodies?

The Western blot technique has many steps, and often the whole procedure may require several hours before result can be recorded. 

An enzyme, like alkaline phosphatase (ALP) or horse radish peroxidase (HRP), or a fluorescent dye, like DyLight®, attached directly to a primary antibody allows shortening of the protocol by many incubation steps. Incubation with a secondary antibody and washes are to be omitted when directly conjugated primary antibodies are used. 

Primary antibodies can be easily conjugated, as available kits are very well optimized to be time and labelling efficient. This approach is recommended for detection of proteins of high and moderate expression. 

Agrisera's collection of directly conjugated primary antibodies to various plant and algal proteins can be found here. 

 		 How to save time in Western blot?
 Technical tips

Technical tips

Latest

Calclulated and aparent molecular weight of detected protein is different, why?
2025-10-09 How to chose right loading control for Western blot?
2025-10-06 Is an antibody going to work in a technique I am planning to use it in?
2025-09-30 Antibody reactivity to recombinant protein,does not validate antibody specificity in endogenous sample
2025-08-20 Can aggregated antibody be still used?
2025-08-15 Blot Once, Probe Twice - when such approach can be beneficial?
2025-07-31 Why should glycerol not be added, to stabilize antibodies in serum?
2025-06-27 Can methanol in transfer buffer be replaced by a less toxic alternative?
2025-05-12 Will the precipitation of SDS in the stripping buffer affect antibody stripping efficiency?
2025-04-29 How to check if protein transfer from a gel to a membrane was efficient
2025-04-15

Archive


Close
Login / Retailer
Register
Forgot password?