SPSA1 | Sucrose phosphate synthase isoform A1

AS15 2995 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPSA1 sequence, Uniprot: Q56X42, TAIR: AT5G20280

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS, pH 7.4

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Expected | apparent MW

117.3 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Arabidopsis alpina, Brassica napus, Eutremas alsugineum, Glycine max, Glycine soja
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Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application example

Western blot using anti-SPSA1 antibody

Proteins were isolated from Arabidopsis thaliana wilde-type and SPS mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 12 µg of proteins were loaded into each lane and separated on 8% SDS-PAGE, and then blotted overnight onto PVDF membrane. Membranes were blocked with milk powder for 2 h and then incubated in the primary antibody solution (AS01 004, 1: 1 000) overnight, which was then decanted and the membrane was washed 3 times for 5 min in TBST. Membrane was incubated at RT for 1 hour in 1:10 000 goat anti-Rabbit secondary antibody (AS09 602), followed by washing steps as above. Membrane was developed for 2 min with Agrisera ECL Bright according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 10 s increment time for around 5 min.

Additional information

Related products

Secondary antibodies

Background

SPSA1 catalyzes the rate-limiting step of sucrose biosynthesis from UDP-glucose and fructose-6-phosphate. It plays a role in regulation of carbon partitioning in the leaves of plants. Furthermore, it is important for sucrose availability which is essential for plant growth and fiber elongation. SPSA1 is also necessary for nectar secretion.

Alternative names: Sucrose-phosphate synthase 1, SPS1, Sucrose-phosphate synthase 1F, SPS1F, Sucrose-phosphate synthase 5.1, SPS5.1, UDP-glucose-fructose-phosphate glucosyltransferase