SPSA1 | Sucrose phosphate synthase isoform A1

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPSA1 sequence, Uniprot: Q56X42, TAIR: AT5G20280
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum in PBS, pH 7.4
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Expected | apparent MW
117.3 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Arabidopsis alpina, Brassica napus, Eutremas alsugineum, Glycine max, Glycine soja
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
Application example


Proteins were isolated from Arabidopsis thaliana wilde-type and SPS mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 12 µg of proteins were loaded into each lane and separated on 8% SDS-PAGE, and then blotted overnight onto PVDF membrane. Membranes were blocked with milk powder for 2 h and then incubated in the primary antibody solution (AS01 004, 1: 1 000) overnight, which was then decanted and the membrane was washed 3 times for 5 min in TBST. Membrane was incubated at RT for 1 hour in 1:10 000 goat anti-Rabbit secondary antibody (AS09 602), followed by washing steps as above. Membrane was developed for 2 min with Agrisera ECL Bright according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 10 s increment time for around 5 min.
Additional information
Background
Background
SPSA1 catalyzes the rate-limiting step of sucrose biosynthesis from UDP-glucose and fructose-6-phosphate. It plays a role in regulation of carbon partitioning in the leaves of plants. Furthermore, it is important for sucrose availability which is essential for plant growth and fiber elongation. SPSA1 is also necessary for nectar secretion.
Alternative names: Sucrose-phosphate synthase 1, SPS1, Sucrose-phosphate synthase 1F, SPS1F, Sucrose-phosphate synthase 5.1, SPS5.1, UDP-glucose-fructose-phosphate glucosyltransferase
Product citations
Related products: SPSA1 | Sucrose phosphate synthase isoform A1
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
This product can b...
This product can b...
From 25 €
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
Off...
Off...
194 €
AS06 185 | Clonality: Polyclonal | Host: Rabbit | Reactivity: ...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
AS03 035A | Clonality: Polyclonal | Host: Rabbit | Reactivity: [glob...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items