2 | Set of 4 Chlamydomonas anti-Lhc antibodies

AS01 011 Chlamydomonas | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

This product includes following antibodies:

AS01 003 Lhcb2 | LHCII type II chlorophyll a/b-binding protein

AS06 117 Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas

AS09 407 Lhcb5 | CP26 (Lhcb5) homolog, Chlamydomonas

AS09 408 Lhcbm5 | chlorophyll a-b binding protein of LHCII

Product Information

Immunogen

Lhcb2 - BSA-conjugated peptide

Lhcb4 - KLH-conjugated synthetic peptide derived from Lhcb4 (CP29, Lhcbm4) protein sequence from Chlamydomonas reinhardtii (Q93WD2)

Lhcb5 - KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii Lhcb5 protein sequence (Q9FEK6)

Lhcbm5 - SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions

Host Rabbit

Clonality Polyclonal

Purity Total IgG in PBS pH 7.4 (Lhcb2), serum (Lhcb4, Lhcb5, Lhcbm5)

Format Lyophilized

Quantity 50 µl (Lhcb5, Lhcbm5), 100 µl (Lhcb4), 200 µl (Lhcb2)

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)

Recommended dilution 1 : 5000 (Lhcb2,Lhcb5, Lhcbm5) (WB) 1 : 10 000 (Lhcb4) (WB)

Expected | apparent MW

25 kDa (Lhcb2)

29 kDa (Lhcb4)

29 | 30 kDa (Lhcbm5)

30| 26 kDa (Lhcb5)

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity Algae (Lhcb2), Angiosperms, Dictos, Gymnosperms, Mosses

Not reactive in

Lhcb4 - higher plants (use AS04 045 for those organsims), algae, cyanobacteria

Application examples

Additional information

Related products

other antibodies against pigment-binding proteins LHC

Plant protein extraction buffer

Secondary antibodies

Background

The outer light-harvesting antenna of the photosystems (PSI and PSII) of the green unicellular alga Chlamydomonas reinhardtii is composed of pigment-binding proteins belonging to the Lhc family highly conserved in photosynthetic eukaryotes. Some of the Lhc-subtypes of Chlamydomonas share sufficient high similarity with the respective functional homologs in plants to allow specific detection with antisera generated against conserved peptide domains from plant Lhc-proteins.

Product citations

Selected references Lhcb2
Du et al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446.

Lhcb4
Jeong et al. (2017). Deletion of the chloroplast LTD protein impedes LHCI import and PSI-LHCI assembly in Chlamydomonas reinhardtii. J Exp Bot. 2017 Dec 30. doi: 10.1093/jxb/erx457.

Lhcb5 and Lhcbm5
Takahashi et al. (2006). Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii. PNAS 103:477-482

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