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Goat anti-Mouse IgG (H&L), HRP conjugated
- Product Info
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Immunogen: Purified mouse IgG (H&L), whole molecule, AAA51107 Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily. Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: The optimal working dilution should be determined by the investigator - Reactivity
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Confirmed reactivity: Heavy chains on mouse IgG and light chains on all mouse immunoglobulins Not reactive in: Non-immunoglobulin mouse serum proteins - Application Examples
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application example
500 femtomoles of His-tagged proteins IsiA, NifH, PsbA, PsbB and PetC were loaded per gel well in Agrisera PEB extraction buffer. Proteins were separated on 4-12 % NuPAGE PAGE Bis-Tris polycacrylamide gel (Invitrogen) and blotted 1h to PVDF. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat, anti-mouse IgG horse radish peroxidase conjugated, from Agrisera AS11 1772) diluted to 1:25 000 in 2 % ECL Advance blocking reagent for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 5 seconds.
Apparent molecular weight of recombinant proteins: IsiA - 27 kda, NifH - 34 kDa, PsbA - 30-37 kDa, PsbB - 40 kDa, PetC - 23 kDa.
- Additional Information
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Additional information: Concentration: 1.0 mg/ml. Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) ProClin 150 as preservative of bacterial growth.
The antibody will detect all isotypes of mouse IgG.
- Background
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Background: Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase mouse IgG (H&L). - Product Citations
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Selected references: Hemmel et al. (2024). Optimized transgene expression in the red alga Porphyridium purpureum and efficient recombinant protein secretion into the culture medium.Plant Mol Biol. 2024 Feb 14;114(1):18.doi: 10.1007/s11103-024-01415-2.
Chung et al. (2023). An RNA thermometer in the chloroplast genome of Chlamydomonas facilitates temperature-controlled gene expression. Nucleic Acids Res. 2023 Nov 10;51(20):11386-11400. doi: 10.1093/nar/gkad816.
Kasari et al. (2019). A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling. Nucleic Acids Res. 2019 Jul 12. pii: gkz600. doi: 10.1093/nar/gkz600.
Li and Bock (2018). Replication of bacterial plasmids in the nucleus of the red alga Porphyridium purpureum. Nat Commun. 2018 Aug 27;9(1):3451. doi: 10.1038/s41467-018-05651-1.
Shin et al. (2017), Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris. Sci Rep. 2017 Dec 20;7(1):17929. doi: 10.1038/s41598-017-18221-0.
Dmitrović et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64. - Protocols
- Antibody protocols
- Reviews:
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Dominika Kamrowska | 2018-09-11Used antibodies to develop Western blot. FLAG tag proteins were co-purified with proteins tagged and purified on MYC, obtained from leaf extract (N benthamiana). Used concentration 1:5000. Bands were quickly and clearly visible.
Accessories
AS10 1020 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG Fc part
AS10 1066 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG (H&L)
AS10 962 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG (H&L)